Behavioral assessment of rats through the forced-swimming test
DOI:
https://doi.org/10.15448/1980-6108.2015.1.20113Keywords:
Behavior, animal, Exercise, Stress, psychologicalAbstract
Aims: Regular exercise has several beneficial effects. However, reports show increasing concentrations of endogenous corticosteroids, which may inhibit neurogenesis and deteriorate cognitive functions, thus raising doubts as to the benefits of physical exercise. The objective of this study was to evaluate, through behavioral tests, the effect of chronic forced swimming with and without overload on anxiety parameters in Wistar rats.
Methods: Twenty-four Wistar rats, divided into three groups, were assessed: Control Group – subjected only to evaluations and contact with water; Swimming Group – consisting of animals submitted to progressive swimming time; Weight-loaded Swimming Group – consisting of animals submitted to swimming with loads in excess of 10% of their body weight. The exercise was performed three times a week in the afternoon, with swimming time increases every two weeks (20, 30, and 40 minutes), during six weeks, A 200-liter circular water tank, filled to a depth of 50 cm, was used, and the water temperature was kept between 30º and 32º C. The animals’ anxiety was assessed by the Elevated Plus Maze and Open Field tests.
Results: No significant differences between the three groups were detected by any of the behavioral tests.
Conclusions: No changes were observed in the behavioral pattern of Wistar rats submitted to different exercise protocols (forced swimming with and without overload) compared to the control group.
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References
Ciolac EG, Guimarães GV. Exercício físico e síndrome metabólica. Rev Bras Med Esporte. 2004;10(4):319-24. http://dx.doi.org/10.1590/S1517-86922004000400009
Liebetanz D, Gerber J, Schiffner C, Schütze S, Klinker F, Jarry H, et al. Pre-infection physical exercise decreases mortality and stimulates neurogenesis in bacterial meningitis. J Neuroinflammation. 2012;9:168. http://dx.doi.org/10.1186/1742-2094-9-168
Langlois F, Tuong T, Vu M, Chassé K, Dupuis G, Kergoat M, et al. Benefits of physical exercise training on cognition and quality of life in frail older adults. J Gerontol B Psychol Sci Soc Sci. 2012;68(3):400-4. http://dx.doi.org/10.1093/geronb/gbs069
Cheik NC, Reis IT. Efeitos do exercício físico e da atividade física na depressão e ansiedade em indivíduos idosos. R Bras Ci e Mov. 2003;11(3):45-51.
Benite-Ribeiro SA, Matzenbacher J, Duarte JAR. Moderate physical exercise attenuates the alterations of feeding behaviour induced by social stress in female rats. Cell Biochem Funct. 2014;32(4):142-9. http://dx.doi.org/10.1002/cbf.2984
Calik MW, Shankarappa SA, Stubbs EB. Forced-exercise attenuates experimental autoimmune neuritis. Neurochem Int. 2012;61(2):141-5. http://dx.doi.org/10.1016/j.neuint.2012.04.023
Di Luigi L, Botrè F, Sabatini S, Sansone M, Mazzarino M, Guidetti L, Baldari C, Lenzi A, Caporossi D, Romanelli F, Sgrò P. Acute effects of physical exercise and phosphodiesterase's type 5 inhibition on serum 11b-hydroxysteroid dehydrogenases related glucocorticoids metabolites: a pilot study. Endocrine. 47(3):952-8. http://dx.doi.org/10.1007/s12020-014-0185-2
Navarro F, Bacurau AVN, Almeida SS, Barros CC, Moraes MR, Pesquero JL, Ribeiro SM, Araújo RC, Costa Rosa LF, Bacurau RF. Exercise prevents the effects of experimental arthritis on the metabolism and function of immune cells. Cell Biochem Funct. 2010;28(4):266-73. http://dx.doi.org/10.1002/cbf.1647
Cherem EHL, Santos LC dos, Azeredo FP de, Serra RA, Sá CCNF de. Alteração da testosterona, cortisol, força e massa magra após 20 semanas como resposta a três metodologias de treinamento de força. Rev Bras Fisiol Ex. 2014;13(4):188-96.
Kuphal KE, Fibuch EE, Taylor BK. Extended swimming exercise reduces inflammatory and peripheral neuropathic pain in rodents. J Pain. 2007;8(12):989-97. http://dx.doi.org/10.1016/j.jpain.2007.08.001
Bertolini GRF, Rosa CT, Silva LI, Meireles A, Rocha BP. Uso do exercício resistido antagonizado por naloxone como fator de analgesia em sinovite aguda de joelho de ratos Wistar. Rev Bras Med Esporte. 2012;18(2):126-9. http://dx.doi.org/10.1590/S1517-86922012000200013
Bement MKH, Sluka KA. Low-intensity exercise reverses chronic muscle pain in the rat in a naloxone-dependent manner. Arch Phys Med Rehabil. 2005;86(9):1736-40. http://dx.doi.org/10.1016/j.apmr.2005.03.029
Hua S, Cabot PJ. Mechanisms of peripheral immune-cell-mediated analgesia in inflammation: clinical and therapeutic implications. Trends Pharmacol Sci. 2010;31(9):427-33. http://dx.doi.org/10.1016/j.tips.2010.05.008
Liu W, Sheng H, Xu Y, Liu Y, Lu J, Ni X. Swimming exercise ameliorates depression-like behavior in chronically stressed rats: Relevant to proinflammatory cytokines and IDO activation. Behav Brain Res. 2013;242:110-6. http://dx.doi.org/10.1016/j.bbr.2012.12.041
Liu W, Xu Y, Lu J, Zhang Y, Sheng H, Ni X. Swimming exercise ameliorates depression-like behaviors induced by prenatal exposure to glucocorticoids in rats. Neuroscie Lett. 2012;524(2):119-23. http://dx.doi.org/10.1016/j.neulet.2012.07.011
Bertolini GRF, Matos CMP, Artifon EL, Ferrari D, Vituri RF. Avaliação funcional da nocicepção do joelho de ratos tratada com laser de baixa potência e natação. Rev Bras Med Esporte. 2011;17(1):45-8. http://dx.doi.org/10.1590/S1517-86922011000100009
Koltyn KF, Umeda M. Exercise, hypoalgesia and blood pressure. Sport Med. 2006;36(3):207-14. http://dx.doi.org/10.2165/00007256-200636030-00003
Fuss J, Abdallah NMB, Vogt MA, Touma C, Pacifici PG, Palme R, Witzemann V, Hellweg R, Gass P. Voluntary exercise induces anxiety-like behavior in adult C57BL/6J mice correlating with hippocampal neurogenesis. Hippocampus. 2010;20(3):364-76. http://dx.doi.org/10.1002/hipo.20634
Li H, Liang A, Guan F, Fan R, Chi L, Yang B. Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress. Brain Res. 2013;1531:1-8. http://dx.doi.org/10.1016/j.brainres.2013.07.041
Walsh RN, Cummins RA. The open field test: a critical review. Psychol Bull. 1976;83(3):482-504. http://dx.doi.org/10.1037/0033-2909.83.3.482
Pellow S, Chopin P, File SE, Briley M. Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods. 1985;14(3):149-67. http://dx.doi.org/10.1016/0165-0270(85)90031-7
Pietá Dias C, Martins de Lima MN, Presti-Torres J, Dornelles A, Garcia VA, Siciliani Scalco F, Rewsaat Guimarães M, Constantino L, Budni P, Dal-Pizzol F, Schröder N. Memantine reduces oxidative damage and enhances long-term recognition memory in aged rats. Neuroscience. 2007;146(4):1719-25. http://dx.doi.org/10.1016/j.neuroscience.2007.03.018
De Lacerda GFM. Ansiedade em modelos animais: efeito de drogas nas dimensões extraídas da análise fatorial [dissertation]. [Curitiba]: Universidade Federal do Paraná; 2006. 62 p. Available from: http://dspace.c3sl.ufpr.br:8080/dspace/handle/1884/3780
Choleris E, Thomas AW, Kavaliers M, Prato FS. A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neurosci Biobehav Rev. 2001;25(3):235-60. http://dx.doi.org/10.1016/S0149-7634(01)00011-2
Smilios I, Pilianidis T, Karamouzis M, Tokmakidis SP. Hormonal responses after various resistance exercise protocols. Med Sci Sport Exerc. 2003;35(4):644-54. http://dx.doi.org/10.1249/01.MSS.0000058366.04460.5F
Handley SL, McBlane JW. 5HT drugs in animal models of anxiety. Psychopharmacology. 1993;112(1):13-20. http://dx.doi.org/10.1007/BF02247358
Bertoglio LJ, Carobrez AP. Previous maze experience required to increase open arms avoidance in rats submitted to the elevated plus-maze model of anxiety. Behav Brain Res. 2000;108(2):197-203. http://dx.doi.org/10.1016/S0166-4328(99)00148-5
File SE, Zangrossi Jr H. "One-trial tolerance" to the anxiolytic actions of benzodiazepines in the elevated plus-maze, or the development of a phobic state? Psychopharmacology (Berl). 1993;110(1-2):240-4. http://dx.doi.org/10.1007/BF02246980
Andersen ML, Bignotto M, Machado RB, Tufik S. Different stress modalities result in distinct steroid hormone responses by male rats. Braz J Med Biol Res. 2004;37(6):791-7. http://dx.doi.org/10.1590/S0100-879X2004000600003
Gavrilovic L, Stojiljkovic V, Kasapovic J, Popovic N, Pajovic SB, Dronjak S. Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats. An Acad Bras Cienc. 2013;85(3):999-1012. http://dx.doi.org/10.1590/S0001-37652013005000041
Filipovic D, Gavrilovic L, Dronjak S, Radojcic MB. The effect of repeated physical exercise on hippocampus and brain cortex in stressed rats. Ann N Y Acad Sci. 2007;1096:207-19. http://dx.doi.org/10.1196/annals.1397.087
Patki G, Li L, Allam F, Solanki N, Dao AT, Alkadhi K, et al. Moderate treadmill exercise rescues anxiety and depression-like behavior as well as memory impairment in a rat model of posttraumatic stress disorder. Physiol Behav. 2014;130:47-53. http://dx.doi.org/10.1016/j.physbeh.2014.03.016
Waters RP, Renner KJ, Summers CH, Watt MJ, Forster GL, Koch LG, et al. Selection for intrinsic endurance modifies endocrine stress responsiveness. Brain Res. 2010;1357:53-61. http://dx.doi.org/10.1016/j.brainres.2010.07.078
Martins LCX, Lopes CS. Rank, job stress, psychological distress and physical activity among military personnel. BMC Public Health. 2013;13:716. http://dx.doi.org/10.1186/14712458-13-716
Sasse SK, Greenwood BN, Masini C, Nyhuis TJ, Fleshner M, Day HEW, et al. Chronic voluntary wheel running facilitates corticosterone response habituation to repeated audiogenic stress exposure in male rats. Stress. 2008;11(6):425-37. http://dx.doi.org/10.1080/10253890801887453
Liu W, Zhou C. Corticosterone reduces brain mitochondrial function and expression of mitofusin, BDNF in depression-like rodents regardless of exercise preconditioning. Psychoneuroendocrinology. 2012;37(7):1057-70. http://dx.doi.org/10.1016/j.psyneuen.2011.12.003