Antioxidant potential of Carica papaya Linn (Caricaceae) leaf extract in mice with cyclophosphamide induced oxidative stress

Keywords: antimutagenicity, antioxidant defense, ethnobotany, secondary metabolites, erythropoiesis


AIMS: This study aimed to investigate the effects of crude extract of Carica papaya leaves on oxidative stress in mice induced by cyclophosphamide, as well as phytochemical profile characterization of this extract.
METHODS: The male Swiss mice received 15 days of treatment with the extract (500 mg kg-1, via gavage) and intraperitoneal injection of cyclophosphamide (75 mg kg-1) or saline (0.9%) on the 15th day. After 24 h the last treatment, the animals were anesthetized for blood withdrawal, sacrificed and removal of the organs for analyses (liver, kidney and heart). In the biochemical tests were determined: hematological parameters in blood, aminotransferases, alkaline phosphatase, glucose and total cholesterol dosages in plasma, enzymatic and non-enzymatic antioxidants and lipid damage marker were evaluated in different tissues, besides genotoxic and histopathological analyzes.
RESULTS: In the extract of Carica papaya leaves, the flavonoids quercetin-3β-D-glucoside and rutin were identified, besides present positive results for alkaloids, saponins and tannins. This extract increased the activity of glutathione-S-transferase and catalase enzymes in the liver and reduced the levels of reduced glutathione in the kidneys and hematocrit levels, red cell count, and hemoglobin. It promoted the decrease of the reactive species of thiobarbituric acid (TBARS) in the kidneys and the activity of enzyme aspartate aminotransferase in the plasma and was antimutagenic in the micronucleus test.
CONCLUSIONS: The study showed that extract of Carica papaya was beneficial against oxidative events and prevented DNA damage. The extract also showed hepatotoxicity, therefore prolonged infusion of papaya leaves is not advisable.


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Author Biographies

Tatiane Cordeiro Luiz, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

MD in Environmental Sciences from the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil), professor of the state (Sinop, MT, Brazil).

Ana Paula Simões da Cunha, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

Master’s Degree student in Environmental Sciences postgraduate program at the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil).

Danilo Henrique Aguiar, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

PhD in Cellular and Structural Biology from the State University of Campinas (UNICAMP, Campinas, SP, Brazil), Professor at the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil).

Marina Mariko Sugui, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

PhD in Pathology from São Paulo State University Júlio de Mesquita Filho (UNESP, Botucatu, SP, Brazil), Professor and collaborating professor of the Environmental Sciences post graduate program at the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil).

Rogério de Campos Bicudo, Empresa Brasileira de Pesquisas Agropecuárias (EMBRAPA) Agrossilvipastoril, Sinop, MT, Brazil

PhD in Analytical Chemistry from São Paulo University (USP, São Carlos, SP, Brasil), Analyst A at Embrapa Agrossilvipastoril in the laboratory management area (Embrapa, Sinop, MT, Brasil).

Adilson Paulo Sinhorin, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

PhD in Chemistry from the Federal University of Santa Maria (UFSM, Santa Maria, RS, Brazil), Permanent Professor of the Environmental Sciences post graduate program at the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil).

Valéria Dornelles Gindri Sinhorin, Federal University of Mato Grosso, Campus of Sinop, Mato Grosso, Brazil

PhD in Toxicological Biochemistry from the Federal University of Santa Maria (UFSM, Santa Maria, RS, Brazil), Permanent Professor of the Environmental Sciences post graduate program at the Federal University of Mato Grosso (UFMT, Sinop, MT, Brazil).


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How to Cite
Luiz, T. C., da Cunha, A. P. S., Aguiar, D. H., Sugui, M. M., Bicudo, R. de C., Sinhorin, A. P., & Sinhorin, V. D. G. (2020). Antioxidant potential of Carica papaya Linn (Caricaceae) leaf extract in mice with cyclophosphamide induced oxidative stress. Scientia Medica, 30(1), e34702.
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