Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus <b>[Abstract in English]</b>
Keywords:
Diagnóstico molecular. Poliomavírus. Imunossuprimidos. ImunocomprometidosAbstract
Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.Downloads
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