In vitro evaluation of the biological potential of <i>Salvia officinalis</i> L. in tumor cells <br><b>[Abstract in English]</b>

Abstract

AIMS: To determine cell viability compared to the biological effect of Salvia officinalis L. extract on cultured tumor cells of the larynx (Hep-2) and in human hepatoma cells (HepG2) METHODS: Tumor cells Hep-2 and HepG2 were grown in DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% inactivated fetal bovine serum and 1% antibiotics (Penicillin/Streptomycin), in incubator at 37°C and humidified with 5% CO2. In the second step they were plated (5 x 104 cells/mL) in 96 well plates during 24 hours to obtain 60-70% confluency, and treated with the hydroalcoholic extract of Salvia officinalis L., negative control with 80% ethanol (v/v) and positive control of hydrogen peroxide (H2O2), t-butyl peroxide hidroxy (TBO), doxorubicin and cisplatin. Cell viability was determined by MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Results were analyzed by Tukey test (p≤0,05) using the SPSS v.19. RESULTS: the hydroalcoholic extract of Salvia officinalis L. showed cytotoxic activity to tumor cells Hep-2 IC50 0,38 ± 0.02 mg/mL for HepG2 IC50 0,54 ± 0.03 mg/mL compared to negative control, staying above the IC50 obtained for their positive controls. Cisplatin showed IC50 below the extract of sage, but to obtain their IC50 was increased exposure time in six hours. CONCLUSIONS: the results of this study suggest that the extract of Salvia officinalis L. has biological activity against tumor cell; further studies are needed to confirm its efficacy and its potential role in cancer treatment.