Effect of resistance training on the reduction of edema, nociception, and nerve regeneration in a sciatic nerve compression model

Authors

  • Juliana Sobral Antunes
  • Keli Lovison
  • Jhenifer Karvat
  • Lisyana Vieira
  • Ana Luiza Peretti
  • Guilherme Hideaki Higuchi
  • Maria Lúcia Bonfleur Universidade Estadual do Oeste do Paraná (Unioeste).
  • Rose Meire Costa Brancalhão
  • Lucinéia de Fátima Chasko Ribeiro
  • Gladson Ricardo Flor Bertolini Universidade Estadual do Oeste do Paraná (Unioeste).

DOI:

https://doi.org/10.15448/1980-6108.2016.3.23711

Keywords:

motor activity, sciatic nerve, sciatic neuropathy, pain measurement, nerve regeneration.

Abstract

Aims: To analyze the effects of ladder-climbing resistance training exercise on edema, nociception, and regeneration of the sciatic nerve in Wistar rats subjected to sciatic nerve compression.

Methods: Twenty-four Wistar rats were divided into four groups: Control Group, Exercise Group, Injury Group, and Treated Group (injury and exercise). Injury Group and Treated Group were subjected to sciatic nerve compression with a hemostat for 30 seconds. On the third day after injury, Exercise Group and Treated Group began treatment with ladder-climbing resistance exercise. The treatment consisted in performing two series of 10 ladder climbs with a 100-gram overload and a one-minute interval between the series. The study was conducted for 22 days, during which time the animals were evaluated for edema and nociception. Twenty-two days after surgery, the animals were anesthetized for removal of a sciatic nerve fragment and analysis of the number of axons and fiber density. Thereafter, still under anesthesia, the animals were euthanized. Nerve sampling followed the routine histological processing protocol. Expressions of Neural Growth Factor and Brain-derived Neurotrophic Factor were evaluated by Western blotting.

Results: There was no significant difference in edema size between groups. Control Group showed the highest nociceptive threshold compared to the other groups. The morphometric analysis showed no significant difference in number of axons and fiber density between groups. The expression of Brain-derived Neurotrophic Factor was greater in the Injury Group and the Treated Group compared to the Control Group.

Conclusions: The proposed ladder-climbing resistance training was not effective in reducing edema and nociception or in increasing the number of axons and fiber density after sciatic nerve injury.

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Author Biographies

Maria Lúcia Bonfleur, Universidade Estadual do Oeste do Paraná (Unioeste).

Doutor (a). Docente do Programa de Pós-Graduação em Biociências e Saúde da Unioeste

Gladson Ricardo Flor Bertolini, Universidade Estadual do Oeste do Paraná (Unioeste).

Possui graduação em Fisioterapia pela Universidade Estadual de Londrina (1995), mestrado em Engenharia Elétrica e Informática Industrial pela Universidade Tecnológica Federal do Paraná (2003) e doutorado em Ciências da Saúde Aplicadas ao Aparelho Locomotor pela Faculdade de Medicina de Ribeirão Preto / Universidade de São Paulo (2008). Atualmente é prof. adjunto C da Universidade Estadual do Oeste do Paraná, docente da graduação em Fisioterapia e Mestrado em Biociências e Saúde. Tem experiência na área de Fisioterapia, com ênfase em Fisioterapia Ortopédica e Traumatológica, atuando principalmente nos seguintes temas: fisioterapia, laser de baixa potência, alongamento muscular, imobilização e remobilização, dor e ultrassom terapêutico. Atualmente é Bolsista Produtividade da Fundação Araucária. Membro do Comitê Assessor de Área - Fisioterapia INEP (MEC). Plantonista do Centro de Reabiltiação Física da Unioeste, com atendimento em Fisioterapia Ortopédica e Traumatológica.

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Published

2016-10-30

How to Cite

Antunes, J. S., Lovison, K., Karvat, J., Vieira, L., Peretti, A. L., Higuchi, G. H., Bonfleur, M. L., Brancalhão, R. M. C., Ribeiro, L. de F. C., & Bertolini, G. R. F. (2016). Effect of resistance training on the reduction of edema, nociception, and nerve regeneration in a sciatic nerve compression model. Scientia Medica, 26(3), ID23711. https://doi.org/10.15448/1980-6108.2016.3.23711

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Vol. 26 No. 3 (2016): Special issue on Metabolic Syndrome

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