Detection of fibronectin and laminin in <i>Toxoplasma gondii</i> tissue cysts: Immunocytochemical assays

Authors

  • Erick Vaz Guimarães Instituto Oswaldo Cruz-Fiocruz
  • Mariana Acquarone Instituto Oswaldo Cruz-Fiocruz
  • Laís de Carvalho Universidade do Estado do Rio de Janeiro
  • Helene Santos Barbosa Instituto Oswaldo Cruz-Fiocruz

Keywords:

Toxoplasma gondii, TISSUE CYSTS, BRADYZOITES, IMMUNOCYTOCHEMISTRY, LAMININ, FIBRONECTIN.

Abstract

AIMS: To analyze the existence and distribution of some matrix proteins in tissue cysts of Toxoplasma gondii. METHODS: Laminin and fibronectin in tissue cysts of Toxoplasma gondii were detected by confocal microscopy and transmission electron microscopy. RESULTS: Ultrastructural immunocytochemistry showed both glycoproteins in the granular region of tissue cysts, cystic matrix, micronemes, rhoptries, dense granules and rarely at the membrane of bradyzoites of Toxoplasma gondii. CONCLUSIONS: The presence of both laminin and fibronectin in secretory organelles and in the apical region of bradyzoites suggests that exocytosis of these glycoproteins can contribute to their interaction with host cells, besides composing the cyst matrix of Toxoplasma gondii.

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Author Biography

Erick Vaz Guimarães, Instituto Oswaldo Cruz-Fiocruz

Possui graduação em Ciências Biológicas pela Universidade Federal do Rio de Janeiro (1995-1999), Mestrado em Biologia Celular e Molecular pela Fundação Oswaldo Cruz (2000-2002) e Doutorado em Biologia Celular e Molecular pela Fundação Oswaldo Cruz (2003-2007). Tem experiência na áreas de Morfologia, com ênfase em Ultra-estrtura Celular; Parasitologia, atuando principalmente nos seguintes temas: Toxoplasma gondii, interação parasito-célula hospedeira, interconversão/cistogênese, ultra-estrutura, citoquímica. Atualmente está como Pesquisador Visitante e Chefe Substituto, no Laboratório de Biologia Estrutural do Instituto Oswaldo Cruz - Fiocruz, coordenando e/ou colaborando em projetos na área de Toxoplasmose experimental.

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Published

2010-03-24

How to Cite

Guimarães, E. V., Acquarone, M., Carvalho, L. de, & Barbosa, H. S. (2010). Detection of fibronectin and laminin in <i>Toxoplasma gondii</i> tissue cysts: Immunocytochemical assays. Scientia Medica, 20(1), 83–87. Retrieved from https://revistaseletronicas.pucrs.br/index.php/scientiamedica/article/view/5889

Issue

Vol. 20 No. 1 (2010): Special issue on Toxoplasmosis

Section

Original Articles

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