Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis

Authors

  • Rodrigo Daniel Genske Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo http://orcid.org/0000-0002-0654-1794
  • Eduardo Alexandre Loth Patologia, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná http://orcid.org/0000-0002-9496-7268
  • Vanessa Cecatto Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná http://orcid.org/0000-0003-3888-131X
  • Rinaldo Ferreira Gandra Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná http://orcid.org/0000-0001-9586-7253
  • Cleverson Marcelo Pilatti Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo http://orcid.org/0000-0002-8930-9113
  • Maricília Silva Costa Química Biológica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo http://orcid.org/0000-0002-0141-7241

DOI:

https://doi.org/10.15448/1980-6108.2019.2.32705

Keywords:

Paracocidioidomycosis, Arthritis experimental, laser therapy, Receptors purinergic, Brilliant Blue G.

Abstract

AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.

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Author Biographies

Rodrigo Daniel Genske, Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

Eduardo Alexandre Loth, Patologia, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Patologia, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Vanessa Cecatto, Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Rinaldo Ferreira Gandra, Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Ciências aplicadas à Saúde, Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel, Paraná

Cleverson Marcelo Pilatti, Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

Engenharia Biomédica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

Maricília Silva Costa, Química Biológica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

Química Biológica, Instituto de Pesquisa e Desenvolvimento da Universidade do Vale do Paraíba (UNIVAP). São José dos Campos, São Paulo

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Published

2019-08-22

How to Cite

Genske, R. D., Loth, E. A., Cecatto, V., Gandra, R. F., Pilatti, C. M., & Costa, M. S. (2019). Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis. Scientia Medica, 29(2), e32705. https://doi.org/10.15448/1980-6108.2019.2.32705

Issue

Vol. 29 No. 2 (2019)

Section

Original Articles

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