Evaluation of both neutrophil migration and the relative frequency of CD4+/CD8+ limphocytes in children with Down syndrome and controls

Authors

  • Artur Kautzmann Filho 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Patrícia Dias de Araújo 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Fernanda Kliemann 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Amanda Tavares Mello 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Paula Colling Klein 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Stéfanie Muraro 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Virgínia Tronco 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Liana Antunes 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Bárbara Nery Porto 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Ana Paula Duarte de Souza 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • Leonardo Araújo Pinto 1 Programa de Pós-graduação em Pediatria e Saúde da Criança, Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) 2 Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)

DOI:

https://doi.org/10.15448/1980-6108.2015.1.19235

Keywords:

Down syndrome, Immunity, CD4-CD8 Ratio, Child, Infection.

Abstract

Aims: To evaluate neutrophil migration and the relative frequency of CD4+/CD8+ lymphocytes in children with Down syndrome and in healthy controls.

Methods: This was a case-control study carried out at the Institute of Biomedical Research, affiliated with São Lucas Hospital of the Pontifical Catholic University of Rio Grande do Sul (PUCRS). Patients with Down syndrome were selected by convenience sampling, including all children with Down syndrome aged 3 to 13 years treated at the Pediatric and Otolaryngology Outpatient Clinics of São Lucas Hospital and at Kinder – Center for Children with Special Needs, in Porto Alegre, State of Rio Grande do Sul, Brazil, between January and December 2012. Healthy children without Down syndrome, participants in another ongoing study conducted by Institute of Biomedical Research, were recruited to the control group. Those patients with the largest volume of cells stored in cryotubes were selected. A neutrophil chemotaxis assay and immunophenotyping of CD4+ and CD8+ T cells were performed to evaluate the functionality of the immune response. Associations were assessed by the chi-squared test, Student’s t test, or Mann-Whitney’s test. All tests were bidirectional, and p values less than 0.05 were regarded as statistically significant.

Results: This study included 19 patients (13 with Down syndrome and six controls), with a mean age of 8.13 and 9.83 years, respectively. No significant changes concerning neutrophil migration were observed in the Down syndrome group. However, patients with Down syndrome tended to have a lower rate of CD4+ T cells and a higher rate of CD8+ T cells. The CD4+/CD8+ ratio revealed significant difference between the groups, being lower in patients with Down syndrome.

Conclusions: This study suggests that patients with Down syndrome show a decreased CD4+/CD8+ ratio, which may contribute to the frequent and recurrent infections in these children.

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References

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Published

2015-04-24

How to Cite

Kautzmann Filho, A., Dias de Araújo, P., Kliemann, F., Tavares Mello, A., Colling Klein, P., Muraro, S., Tronco, V., Antunes, L., Nery Porto, B., Duarte de Souza, A. P., & Araújo Pinto, L. (2015). Evaluation of both neutrophil migration and the relative frequency of CD4+/CD8+ limphocytes in children with Down syndrome and controls. Scientia Medica, 25(1), ID19235. https://doi.org/10.15448/1980-6108.2015.1.19235

Issue

Vol. 25 No. 1 (2015)

Section

Original Articles

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