Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus <b>[Abstract in English]</b>

Authors

  • Juliana Tonietto Domingues Acadêmica da Faculdade de Farmácia - PUCRS
  • Fernanda de Paris Bioquímica do Laboratório de Biologia Molecular do Hospital de Clínicas de Po
  • Virginia Minghelli Schmitt Faculdade de Farmácia e Instituto de Pesquisas Biomédicas - PUCRS

Keywords:

Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos

Abstract

Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.

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How to Cite

Domingues, J. T., de Paris, F., & Schmitt, V. M. (2008). Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus <b>[Abstract in English]</b>. Scientia Medica, 17(2), 72–78. Retrieved from https://revistaseletronicas.pucrs.br/index.php/scientiamedica/article/view/1660

Issue

Vol. 17 No. 2 (2007)

Section

Original Articles

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