Antiviral activity of dermaseptin 01 against <i>Dengue virus</i> type 2, <i>Herpes simplex virus</i> type 1 and <i>Vaccinia virus</i><br><b>[Abstract in English]</b>
DOI:
https://doi.org/10.15448/1980-6108.2013.1.12751Keywords:
PRODUCTS WITH ANTIMICROBIAL ACTION, ANTIVIRAL AGENTS, <i>DENGUE VIRUS</i>, <i>VACCINIA VIRUS</i>, <i>HERPES SIMPLEX</i>, ANPHIBIANS.Abstract
AIMS: Determine the in vitro antiviral activity of dermaseptin-1 an antimicrobial peptide discovered in the skin secretion of Phyllomedusa hypochondrialis frogs against Herpes simplex virus type 1, Vaccinia virus and Dengue virus type 2. METHODS: The Peptide dermaseptin-1 was used for the cytotoxic assays using Vero, LLCMK2 and C6/36 cells. The antiviral activity was evaluated using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay for Herpes simplex virus type 1 and Vaccinia virus in VERO cells and for Dengue virus type 2 in LLCMK2 cells. The antiviral activity of dermaseptin-1was also evaluated in C6/36 cells infected with Dengue virus type 2 by cytopathic effect reduction. RESULTS: The 50% cytotoxicity concentration of dermaseptin-1 was 105 µg/ml in insect cells (C6/36) and more than 1000 µg/ml in mammalian cells (VERO and LLCMK2). DS-01 displayed antiviral effect only against Dengue virus type 2 with a 50% effective concentration of 15 µg/ml in C6/36 cells and 60 µg/ml in LLCMK2 cells. CONCLUSIONS: These data suggested that dermaseptin 01 have an in vitro antiviral action against Dengue virus type 2 but not against Herpes simplex virus type 1 or Vaccinia virus.Downloads
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Published
2013-03-30
How to Cite
Cardoso, J. L. M. de S., Soares, M. J. dos S., Leite, J. R. S. A., Malaquias, L. C. C., & Coelho, L. F. L. (2013). Antiviral activity of dermaseptin 01 against <i>Dengue virus</i> type 2, <i>Herpes simplex virus</i> type 1 and <i>Vaccinia virus</i><br><b>[Abstract in English]</b>. Scientia Medica, 23(1), 18–21. https://doi.org/10.15448/1980-6108.2013.1.12751
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