Mecanismo molecular subjacente à propriedade de cicatrização de feridas in vitro do Stryphnodendron adstringens (Mart.)
DOI:
https://doi.org/10.15448/1980-6108.2025.1.46171Palavras-chave:
Polifenois, Cicatrização de Feridas., antioxidante, AntiinflamatóriosResumo
Objetivo: Analisar o mecanismo do extrato hidroalcóolico de barbatimão na indução da cicatrização células dérmicas de fibroblastos (HFF-1), em um modelo in vitro.
Métodos: Foram estudadas aqui duas concentrações de barbatimão utilizadas em pomadas comerciais (B1 = 0,49 mg/mL; B2 = 0,99 mg/mL), e seus efeitos foram avaliados com os seguintes marcadores às 24 e 72 horas após a indução da ferida in vitro: (1) metabolismo oxidativo [oxidação do DNA, lipoperoxidação, carbonilação de proteínas, enzimas antioxidantes (superóxido dismutase, SOD; catalase, CAT; glutationa peroxidase, GPX)]; (2) citocinas pró-inflamatórias (TNF-α, interleucina 6); (3) marcadores apoptóticos [caspase 3 (CASP3), CASP8, e fatores de crescimento: de queratinócito (KGF) e de fibroblastos-1 (FGF-1)].
Resultados: O tratamento com ambas as concentrações mostrou diminuição nos marcadores pró-oxidantes, pró-inflamatórios e pró-apoptóticos, enquanto aumentava os níveis de antioxidantes e fatores de crescimento.
Conclusões: O barbatimão atua por meio de um mecanismo pleiotrópico em cascata que envolve a modulação antioxidante, anti-inflamatória, anti-apoptótica e de migração/proliferação de fibroblastos. Nossos dados poderiam explicar o efeito e a importância do extrato de barbatimão nos mecanismos de cicatrização de feridas.
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Copyright (c) 2025 Neida Luiza Kaspary Pellenz , Moisés Henrique Mastella, Verônica Farina Azzolin , Cibele Ferreira Teixeira, Paula Köhler Carpilovsky, Lucas Muller Brunelli, Cristiane Köhler Carpilovsky, Ijoni Hilda Costabeber, Ivana Beatrice Mânica da Cruz, Marta Maria Medeiros Frescura Duarte, Fernanda Barbisan
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