Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

Paloma Filomena Gouveia Rodrigues; Lucinéia Reuse Albiero; Eduardo Figueredo Nery; Taiany Oliveira Kelly; Jeniffer Charlene Silva Dalazen; Débora Linsbinski Pereira; Adilson Paulo Sinhorin; Valéria Dornelles Gindri Sinhorin; Lindsey Castoldi

doi:10.15448/1980-6108.2019.1.32408

Autores

Paloma Filomena Gouveia Rodrigues Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-1860-9565
Lucinéia Reuse Albiero Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-2899-2262
Eduardo Figueredo Nery Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0001-5006-9450
Taiany Oliveira Kelly Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-3461-767X
Jeniffer Charlene Silva Dalazen Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-7233-5761
Débora Linsbinski Pereira Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-6587-0352
Adilson Paulo Sinhorin Natural, Human, and Social Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-4133-8994
Valéria Dornelles Gindri Sinhorin Natural, Human, and Social Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0002-5070-0043
Lindsey Castoldi Health Sciences Institute, Federal University of Mato Grosso, Sinop, Mato Grosso, Brazil. http://orcid.org/0000-0001-9678-5815

DOI:

https://doi.org/10.15448/1980-6108.2019.1.32408

Palavras-chave:

Copaifera multijuga, fabaceae, copaiba, natural killer cells, carcinoma, Ehrlich tumor, immunomodulation.

Resumo

***Atividade natural killer das células esplênicas de camundongos portadores do tumor de Ehrlich tratados com extrato de Copaifera multijuga***

OBJETIVOS: O óleo-resina de Copaifera multijuga Hayne é popularmente utilizado na medicina tradicional por suas propriedades anti-inflamatória, antisséptica, antitumoral e antibacteriana. Entretanto, há poucos estudos sobre o efeito dos compostos obtidos da casca da planta. Assim, o objetivo deste trabalho foi avaliar o efeito imunomodulador do extrato etanólico da casca da C. multijuga na atividade natural killer das células esplênicas não aderentes de animais portadores do tumor de Ehrlich.

MÉTODOS: Camundongos Swiss machos foram inoculados subcutaneamente com 1 x 10⁶ células do tumor de Ehrlich (grupo Ehrlich e Ehrlich/C. multijuga) ou com solução salina tamponada (grupo controle e C. multijuga) e tratados diariamente (gavagem) com extrato de C. multijuga (200 mg kg^-1, 0,1mL cada um, para os grupos Ehrlich/C. multijuga e C. multijuga) ou com solução salina tamponada (grupo controle e grupo Ehrlich). Os quatro grupos experimentais consistiram de oito animais cada e foram organizados em dois conjuntos, um tratado por sete dias e outro tratado por 14 dias, totalizando 64 animais durante todo o experimento. Após 24 horas do término do tratamento, os animais foram eutanasiados para obtenção da suspensão de células esplênicas não aderentes e avaliação da atividade natural killer. Os resultados são apresentados como porcentagem da lise celular das células alvo Yac.1 pelas células esplênicas não aderentes.

RESULTADOS: A análise dos resultados demonstrou que aos sete dias de tratamento a C. multijuga aumentou a atividade natural killer no grupo Ehrlich/C. multijuga (21,20±8,89, p<0,05) em comparação ao grupo controle (3,14±2,71, p<0,05), entretanto tal efeito não foi mantido nos grupos tratados por 14 dias (Controle: 6,02±6,98; EHR: 4,82±7,72; C. multijuga: 2,07±2,10; EHR/C. multijuga: 2,01±1,63, p>0,05).

CONCLUSÕES: Os resultados demonstraram que o tratamento com o extrato etanólico da casca de C. multijuga favoreceu a atividade natural killer das células esplênicas não aderentes dos animais portadores do tumor de Ehrlich apenas aos sete dias de tratamento.

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