Evaluation of <i>Lawsonia inermis</i> (henna) pigment as a carrier of antigens inoculated by transcutaneous immunization pigment in mice<br><b>[Abstract in English]</b>

  • Karoline Biffi Fac. de Med. de Ribeirão Preto – USP
  • Alexandre Gonçalves de Rezende USP-IPT-Instituto Butantan
  • Letícia de Almeida Universidade Federal de Alfenas
  • Caroline Kimie Brandão Yajima Universidade Federal de Alfenas
  • Fernanda Cinto Barbieri RHEABIOTECH Des. Prod. Com. Prod. de Biotec. Ltda
  • Marcela de Lima Moreira Centro de Pesquisas René Rachou - FIOCRUZ, Belo Horizonte, MG
  • Maria Cristina Lorencini de Britto Universidade Federal de Alfenas
  • Vanessa Lopes Rosa Universidade Federal de Alfenas
  • Ticiane Batista da Silva Universidade Federal de Alfenas
  • Tayra Ferreira Oliveira de Lima Universidade Federal de Alfenas
  • Renata de Oliveira Santos Costa Universidade Federal de Alfenas
  • Pedro Eduardo Nascimento Silva Vasconcelos Universidade Federal de Alfenas
  • Luiz Felipe Leomil Coelho Universidade Federal de Alfenas
  • Luiz Cosme Cotta Malaquias Universidade Federal de Alfenas
Keywords: VACCINATION, TRANSCUTANEOUS ADMINISTRATION, ANTIBODIES, ADJUVANTS, IMMUNOLOGIC, IMMUNOADJUVANTS

Abstract

AIMS: To evaluate the role of pigment Lawsonia inermis (henna) as a carrier of antigens in transcutaneous vaccination in mice. METHODS: Mice were immunized transcutaneously into the skin of the abdomen and the ear’s skin with crude antigen of Paracoccidioides brasiliensis and with bovine serum albumin at concentrations of 1, 10 and 50 mg/mL, three times at one week intervals in the presence or absence of pigments L. inermis. One week after last immunization, the animals were evaluated for the presence of serum antibodies, interleucin-4 and interferon-gamma cytokines, and nitric oxide in culture supernatants. RESULTS: No specific antibodies were detected to P.brasiliensis or bovine serum albumin antigens. There were no significant differences in the production of nitric oxide, interleucin-4 and interferon-gamma in supernatants of cell culture. CONCLUSIONS: Transcutaneous immunization with crude antigen of P. brasiliensis or bovine serum albumin suspended in pigment of L. inermis produced no detectable antigenic response in mice.

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Author Biography

Luiz Cosme Cotta Malaquias, Universidade Federal de Alfenas
Instituto de Ciências Biomédicas. Departamento de Microbiologia e Imunologia
Published
2013-03-30
How to Cite
Biffi, K., de Rezende, A. G., de Almeida, L., Yajima, C. K. B., Barbieri, F. C., Moreira, M. de L., de Britto, M. C. L., Rosa, V. L., da Silva, T. B., de Lima, T. F. O., Costa, R. de O. S., Vasconcelos, P. E. N. S., Coelho, L. F. L., & Malaquias, L. C. C. (2013). Evaluation of <i>Lawsonia inermis</i> (henna) pigment as a carrier of antigens inoculated by transcutaneous immunization pigment in mice<br><b>[Abstract in English]</b>. Scientia Medica, 23(1), 34-40. https://doi.org/10.15448/1980-6108.2013.1.12084
Section
Original Articles