Surface microhardness evaluation of enamel and dentin in bovine and human teeth (permanent and deciduous)

Authors

  • Tiago Aurélio Donassollo Universidade Federal de Pelotas
  • Ana Regina Romano Universidade Federal de Pelotas
  • Flávio Fernando Demarco Universidade Federal de Pelotas
  • Álvaro Della-Bona Universidade de Passo Fundo

Keywords:

microhardness, human teeth, bovine teeth

Abstract

The objective of this study was to evaluate the surface microhardness of enamel (E) and dentin (D) from human deciduous molar teeth (Group d), human permanent molar teeth (Group p) and bovine incisor teeth (Group b). Four specimens were selected for each group and stored in saline solution until testing. The specimens were embedded into a resin matrix and polished to 600 grit SiC paper creating a flat enamel surface. The Knoop hardness test was performed in a microhardness device, using a 200 g load. Five measurements were made in each specimen. After that, specimens were polished to expose dentin (near to dentinenamel junction), and other five microhardness measurements were performed per specimen. The values were calculated and statistically analyzed (ANOVA and Tukey tests). Mean hardness values (kg/mm2) and standard deviations were as follows: Ed 338 ± 30.1; Ep 341 ± 32.8; Eb 326 ± 25.5; Dd 104 ± 9.7; Dp 93.3 ± 8.7; Db 91.2 ± 7.3. The microhardness in enamel (E) was statistically higher than in dentin (D) for the three type of teeth studied (p < 0.001). Considering the type of substrate, there were no statistical differences, meaning that the mean hardness values for either enamel or dentin were not different for the human deciduous teeth, human permanent and bovine teeth. UNITERMS: microhardness; human teeth; bovine teeth.

Author Biographies

Tiago Aurélio Donassollo, Universidade Federal de Pelotas

Mestre e Doutorando em Dentística pela Faculdade de Odontologia da Universidade Federal de Pelotas – RS, Cirurgião Dentista do Exército Brasileiro.

Ana Regina Romano, Universidade Federal de Pelotas

Mestre e Doutora em Odontopediatria pela FOUSP, Professora Adjunta do Departamento de Odontologia social e preventiva da Faculdade de Odontologia da Universidade Federal de Pelotas - RS.

Flávio Fernando Demarco, Universidade Federal de Pelotas

Doutor em Dentística pela FOUSP; Professor Adjunto do Departamento de Odontologia Restauradora da Faculdade de Odontologia da Universidade Federal de Pelotas - RS.

Álvaro Della-Bona, Universidade de Passo Fundo

Doutor em Biomateriais pela Universidade da Flórida, EUA; Professor Titular I do Departamento de Odontologia Restauradora da Faculdade de Odontologia da Universidade de Passo Fundo - RS.

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Published

2007-12-27

Issue

Vol. 22 No. 58 (2007)

Section

Original Article

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