In vitro antimicrobial activity of plants of the Amazon on oral biofilm micro-organisms
DOI:
https://doi.org/10.15448/1980-6523.2015.4.17794Keywords:
Biofilm, Micro-organisms, Medicinal plantsAbstract
Objective: Performing a screening of Amazonian species as antimicrobial action of oral biofilm micro-organisms in vitro.
Methods: Determining the Minimum Inhibitory Concentration (MIC) for solid medium diffusion and Minimum Inhibitory Concentration of Adherence (MICA). The strains tested were S. sobrinus (ATCC 27609), S. mutans (ATCC 25175), S. mitis (ATCC 9811); S. sanguis (ATCC10556), L. casei (ATCC 7469).
Results: Juca, Crajiru, Alfavaca and Copaiba presented MIC with halos of inhibition for all strains tested, with values of 62.5, 500, 1000 and 1000 mg/mL, respectively. In MICA, extracts of Juca, Jambu and Crajiru inhibited the adherence in all strains tested.
Conclusions: Extracts of Juca, Crajiru, Alfavaca and Copaiba essential oil had antimicrobial activity against the strains tested and extracts of Juca, Crajiru, Alfavaca, Jambu and Copaiba and Andiroba oils inhibited microbial adherence.
References
Añez RBS. Análise morfoanatômica das folhas e casca de Aspidosperma nitidum Benth e Aspidosperma marcgravianum Woodson (Apocynaceae) com abordagem farmacognóstica e etnofarmacológica. [tese]. Manaus (AM): Programa de Pós-graduação em Biologia Tropical e recursos naturais, Instituto Nacional de Pesquisas da Amazônia (INPA)/ Universidade Federal do Amazonas (UFAM); 2009.
Bandeira MFCL, Oliveira MRB, Benatti Neto C, Comelli LRC. Estudo preliminar da atividade antibacteriana do óleo essencial e da resina da Copífera multijuga (óleo de copaíba), associado ao óxido de zinco e ao hidróxido de cálcio. J Bras Clín Est Odontol 1999; 3(17): 46-52.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4): 493-6.
Borghi WMMC, Moimaz SAS, Saliba NA. Métodos alternativos para higienização bucal e terapêutica odontológica. Rev Inst Ciênc Saúde 2005; 23(4): 309-14.
Borrás MRL. Plantas da Amazônia: Medicinais ou mágicas? – Plantas comercializadas no mercado Adolpho Lisboa. 1.ed. Manaus: Editora Valer/Governo do Estado do Amazonas; 2003.
Bugno NA, Nicolleti MA, Almodóvar AAB, Pereira TC, Auricchio MT. Enxaguatórios bucais: Avaliação da eficácia antimicrobiana de produtos comercialmente disponíveis. Rev Inst Adolfo Lutz 2006; 65(1): 40-5.
Carvalho JC, Teixeira JR, Souza PJ, Bastos JK, Santos Filho D, Sarti SJ. Preliminary studies of analgesic and anti-inflamatory properties of Caesalpinia ferrea crude extract. J Ethnopharm 1996; 53: 175-8.
Diniz MFFM, Oliveira RAG, Medeiros ACD, Malta-Junior A. Memento Fitoterápico: As plantas como alternativa terapêutica - Conhecimentos populares e científicos. João Pessoa: Universitária/ Universidade Federal da Paraíba (UFPB); 1997.
Domingues FC, Queiroz JA, Cabral JM, Fonseca LP. The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30. Enzyme Microb Technol 2000; 26(5-6): 394-401.
Evangelista SS, Sampaio FC, Parente RC, Bandeira MFCL. Fitoterápicos na Odontologia: Estudo etnobotânico na cidade de Manaus. Rev Bras Plantas Med 2013; 15(4): 513-9.
Gebara ECE, Zardetto, CGDC, Mayer MPA. Estudo in vitro da ação antimicrobiana de substâncias naturais sobre S. mutans e S. sobrinus. Rev Odontol Univ São Paulo 1996; 10(4): 251-6.
Kuster RM, Rocha LM. Cumarinas, cromonas e xantonas. In: Simões CMO et al. Farmacognosia: Da planta ao medicamento. 5.ed. Porto Alegre: Editora UFRGS; 2003. p. 537-56 .
Marreiro RO, Bandeira MFCL, Souza TP, Almeida MC, Bendaham K, Venâncio GN, et al. Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea. Clin, Cosmet Investigat Dent. 2014 Jan; 6: 9-13.
Moreira ACA, Santos TAM, Carneiro MC, Porto MR. Atividade de um enxaguatório bucal com clorexidina a 0,12% sobre a microbiota sacarolítica da saliva. Rev Cienc Méd Biol 2008; 7(3): 266-72.
Moreira NA, Ferreira RC, Vieira PA, Valadares HAC. Agentes antimicrobianos no controle da placa supragengival parte I. Arq Odontol 2001; 37(1): 87-98.
Oliveira ACM, Fontana A, Negrini TC, Nogueira MNM, Bedran TBL, Andrade CR, et al. Emprego do óleo de Melaleuca alternifolia Cheel (Myrtaceae) na Odontologia: Perspectivas quanto à utilização como antimicrobiano alternativo às doenças infecciosas de origem bucal. Rev Bras Pl Med 2011; 13(4): 492-9.
Orellana BJP, Kobayachi ES, Lourenço GM. Terapia alternativa através do uso da andiroba. Lato & Sensu 2004; 5(1): 136-41.
Pereira JV, Pereira MSV, Higino JS, Sampaio FC, Alves PM, Araújo CRF. Estudos com o extrato da Punica granatum Linn. (romã): Efeito antimicrobiano in vitro e avaliação clínica de um dentifrício sobre micro-organismos do biofilme dental. Rev Odonto Ciênc 2005; 20(49): 262-9.
Pereira LP, Silva RO, Bringel PH, Silva KE, Assreuy AM, Pereira MG. Polysaccharide fractions of Caesalpinia ferrea pods: Potential anti-inflammatory usage. J Ethnopharmacol 2012; 139(2): 642-8.
Sampaio FC, Pereira MS, Dias CS, Costa VC, Conde NC, Buzalaf MA. In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens. J Ethnopharmacol 2009; 124(2): 289-94.
Santos RI, Pereira DFA, Teodoro GR, Del Ciampo JO, Ogasawara MS, Canettieri ACV et al. Óleo essencial de Thymus vulgaris: Elaboração de enxaguatório bucal e avaliação do efeito in vitro na formação da placa bacteriana. Lat Am J Pharm 2010; 29(6): 941-7.
Soares DGS, Oliveira CB, Leal C, Drumond MRS, Padilha WWN. Susceptibilidade in vitro de bactérias bucais a tinturas fitoterápicas. Rev Odonto Ciênc 2006; 21(53): 232-7.
Spelman K, Depoix D, McCray M, Mouray E, Grellier P. The traditional medicine Spilanthes acmella, and the alkylamides spilanthol and undeca-2E-ene-8,10-diynoic acid isobutylamide, demonstrate in vitro and in vivo anti-malarial activity. Phytother Res 2011; 25(7): 1098-101.
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