Postmenopausal women with high TNF-α concentrations presented less reduction in fat and blood lipids

A randomized controlled clinical trial

Keywords: weight loss, inflammation, high-protein, physical conditioning

Abstract

Aims: evaluate the effects of a high-protein diet associated with physical exercise on inflammatory markers and body composition. 
Methods: the study is a 12-week clinical trial of 26 postmenopausal women who received an individualized high-protein food plan and participated in three multicomponent training sessions each week. Food intake was monitored through eight 24-hour food recalls, and the habitual food intake was estimated. At the beginning and end of the study, anthropometric variables were measured; fat content and lean mass were estimated using formulas, and blood was collected for C-reactive protein (CRP) quantification, including TNF-α, IL-6, IL-10, and IL-18. One-way ANOVA was performed.
Results: it was identified that 13 participants had a high-protein (HP) diet and 13 had a standard-protein (SP) diet. The HP group lost weight (p = 0.032); however, there were no changes in the fat content, the lean mass content, or the inflammatory markers. Only women who started the program with lower TNF-α values showed significant loss of total fat (p = 0.049), visceral fat (p = 0.037), triglycerides (p = 0.031), and LDL cholesterol (p = 0.003). 
Conclusion: postmenopausal women with high concentrations of inflammatory markers are less responsive to strategies for modifying body composition. 

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Author Biographies

Jamylle Araújo Almeida, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Mestre em Ciências da Nutrição e graduada em Nutrição pela Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil. 

Liliane Viana Pires, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Mestre em Ciências da Nutrição e graduada em Nutrição pela Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil.

Luana Edla Lima, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Mestre em Ciências da Nutrição e graduada em Nutrição pela Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil.

Francismayne Batista Santana, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Especialista em Saúde do Adulto e do Idoso e graduada em Nutrição pela Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil.

Walderi Monteiro da Silva Júnior, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Doutor em Clínica Médica pela Universidade Federal do Rio de Janeiro (UFRJ), no Rio de Janeiro, RJ, Brasil. Mestrado em Engenharia Biomédica e graduado em fisioterapia pela Universidade Federal da Paraíba. Professor Associado da Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil.

Marzo Edir da Silva Grigoletto, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Doutor em “Ciencias Aplicadas a la Actividad Física y el Deporte” pela Universidad de Córdoba, com pós-doutorado no Hospital Reina Sofia. Professor da Universidade Federal de Sergipe, em São Cristóvão, SE, Brasil.

Raquel Simões Mendes Netto, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Doutora e mestre em Ciências dos Alimentos pela Universidade de São Paulo (USP), em São Paulo, SP, Brasil. Graduada em Nutrição pela Universidade Federal de Pernambuco. Professora associada da Universidade Federal de Sergipe (UFS), em São Cristóvão, SE, Brasil.

References

Al-Safi ZA, Polotsky AJ. Obesity and Menopause. Best Pract Res Clin Obstet Gynaecol. 2015;29(4):548-53.

Federação Brasileira das Sociedades de Ginecologia e Obstetrícia. CLIMATÉRIO Manual de Orientação; 1995. 78 p.

Abdulnour J, Doucet É, Brochu M, Lavoie JM, Strychar I, Rabasa-Lhoret R, et al. The effect of the menopausal transition on body composition and cardiometabolic risk factors: A Montreal-Ottawa New Emerging Team group study. Menopause. 2012;19(7):760-7.

Maltais ML, Desroches J, Dionne IJ. Changes in muscle mass and strength after menopause. J Musculoskelet Neuronal Interact. 2009;9(October):186-97.

Castro AM, Macedo-de la Concha LE, Pantoja-Meléndez CA. Low-grade inflammation and its relation to obesity and chronic degenerative diseases. Rev Médica del Hosp Gen México. 2017;80(2):101-5.

Franceschi C, Bonafè M, Valensin S, Olivieri F, De Luca M, Ottaviani E, et al. Inflamm-aging: An Evolutionary Perspective on Immunosenescence. Ann N Y Acad Sci. 2000;908(1):244-54.

Visser M, Pahor M, Taaffe DR, Goodpaster BH, Simonsick EM, Newman AB, et al. Relationship of Interleukin-6 and Tumor Necrosis Factor-α With Muscle Mass and Muscle Strength in Elderly Men and Women: The Health ABC Study. J Gerontol Med Sci Am. 2002;57(5):326-32.

Fisher G, Bickel C, Hunter G. Elevated Circulating TNF-α in Fat-Free Mass Non-Responders Compared to Responders Following Exercise Training in Older Women. Biology (Basel) [Internet]. 2014;3(3):551-9. Available from: http://www.mdpi.com/2079-7737/3/3/551

Peake JM, Kukuljan S, Nowson CA, Sanders K, Daly RM. Inflammatory cytokine responses to progressive resistance training and supplementation with fortified milk in men aged 50+ years: An 18-month randomized controlled trial. Eur J Appl Physiol. 2011;111(12):3079-88.

Orsatti Fl, Nahas Eap, Orsatti Cl, Oliveira Ep De, Nahas-Neto J, Mota Gr Da, Et Al. Muscle Mass Gain After Resistance Training is Inversely Correlated With Trunk Adiposity Gain In Postmenopausal Women. J Strength Cond Res. 2012;26(8):2130-9.

Simpson RJ, Kunz H, Agha N, Graff R. Exercise and the Regulation of Immune Functions [Internet]. 1st ed. Vol. 135, Progress in Molecular Biology and Translational Science. Elsevier Inc.; 2015. p. 355-380. Available from: http://dx.doi.org/10.1016/bs.pmbts.2015.08.001

Forti LN, Van Roie E, Njemini R, Coudyzer W, Beyer I, Delecluse C, et al. Load-Specific Inflammation Mediating Effects of Resistance Training in Older Persons. J Am Med Dir Assoc. 2016;17(6):547-52.

Hagner-Derengowska M, Kałużny K, Hagner W, Plaskiewicz A, Bronisz A, Borkowska A, et al. The effect of a 10-week Nordic walking training program on the level of GH and LH in elderly women. Climacteric [Internet]. 2015;18(6):835-40. Available from: http://www.tandfonline.com/doi/full/10.3109/13697137.2015.1058354

Junior RSM, Paulo de Tarso Maciel-Pinheiro E da MMP, Luiz Felipe da Silva Figueiredo RT, Carneiro LSF, Rodrigues VD, Osvaldo José Moreira Nascimento ACD, et al. Effect of Exercise on Inflammatory Profile of Older Persons: Systematic Review and Meta-Analyses. J Phys Act e Heal. 2017;32:1-44.

Monfort-Pires M, Folchetti LD, Previdelli AN, Siqueira-Catania A, de Barros CR, Ferreira SRG. Healthy Eating Index is associated with certain markers of inflammation and insulin resistance but not with lipid profile in individuals at cardiometabolic risk. Appl Physiol Nutr Metab [Internet]. 2014;39(4):497-502. Available from: http://www.nrcresearchpress.com/doi/abs/10.1139/apnm-2013-0279

Montonen J, Boeing H, Fritsche A, Schleicher E, Joost HG, Schulze MB, et al. Consumption of red meat and whole-grain bread in relation to biomarkers of obesity, inflammation, glucose metabolism and oxidative stress. Eur J Nutr. 2013;52(1):337-45.

Rizzoli R. Nutrition and Sarcopenia. J Clin Densitom. 2015;18(4):483-7.

Deer RR, Volpi E. Protein intake and muscle function in older adults. Curr Opin Clin Nutr Metab Care [Internet]. 2015;18(3):248-53. Available from: http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00075197-201505000-00008

Paddon-Jones D, Leidy H. Dietary protein and muscle in older persons. Curr Opin Clin Nutr Metab Care. 2014;17(1):5-11.

Shlisky JD, Durward CM, Zack MK, Gugger CK, Campbell JK, Nickols-Richardson SM. An energy-reduced dietary pattern, including moderate protein and increased nonfat dairy intake combined with walking promotes beneficial body composition and metabolic changes in women with excess adiposity: a randomized comparative trial. Food Sci Nutr [Internet]. 2015;3(5):376-93. Available from: http://doi.wiley.com/10.1002/fsn3.231

Kim JE, O’Connor LE, Sands LP, Slebodnik MB, Campbell WW. Effects of dietary protein intake on body composition changes after weight loss in older adults: A systematic review and meta-analysis. Nutr Rev. 2016;74(3):210-24.

Hermsdorff HHM, Zulet MÁ, Abete I, Martínez JA. A legume-based hypocaloric diet reduces proinflammatory status and improves metabolic features in overweight/obese subjects. Eur J Nutr. 2011;50(1):61-9.

Lopez-Legarrea P, de la Iglesia R, Abete I, Navas-Carretero S, Martinez JA, Zulet MA. The protein type within a hypocaloric diet affects obesity-related inflammation: The RESMENA project. Nutrition [Internet]. 2014;30(4):424-9. Available from: http://dx.doi.org/10.1016/j.nut.2013.09.009

Daly RM, Connell SLO, Mundell NL, Grimes CA, Dunstan DW, Nowson CA. Protein-enriched diet , with the use of lean red meat , combined with progressive resistance training enhances lean tissue mass and muscle strength and reduces circulating IL-6 concentrations in elderly women : a cluster randomized controlled trial 1 – 4. 2014;899-910.

Erdfelder E, Faul F, Buchner A. GPOWER: A general power analysis program. Behav Res Methods, Instruments, Comput. 1996;28(1):1-11.

Aragão-Santos JC, De Resende-Neto AG, Nogueira AC, Feitosa-Neta M de L, Brandão LH, Chaves LM, et al. The effects of functional and traditional strength training on different strength parameters of elderly women: A randomized and controlled trial. J Sports Med Phys Fitness. 2019;59(3):380-6.

Tomeleri CM, Ribeiro AS, Souza MF, Schiavoni D, Schoenfeld BJ, Venturini D, et al. Resistance training improves inflammatory level, lipid and glycemic profiles in obese older women: A randomized controlled trial. Exp Gerontol. 2016;84:80-7.

WHO. Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep Ser [Internet]. 2000;894:1-253. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11234459

Lipschitz DA. Screening for nutritional status in the elderly. Prim Care [Internet]. 1994;21(1):55–67. Available from: http://www.ncbi.nlm.nih.gov/pubmed/8197257

Frisancho AR. Triceps norms skin fold and for assessment upper arm muscle size of nutritional. Am J Clin Nutr. 1974;27(October):1052-8.

Asgari M, Salamat M, Shanei A, Salamat A, Khoshhali M. Anthropometric predictive equations for estimating body composition. Adv Biomed Res. 2015;4(1):34.

Lohman TG, Roche AF, Martorell R. Anthropometric standardization reference manual. Hum Kinet Books. 1988. 177 p.

(DIfE) D of E of the GI of HNP-R. Plataforma Online - The Multiple Source Method [Internet]. [cited 2018 Jan 3]. Available from: https://msm.dife.de/

Hartting U, Haubrock J, Knuppel S, Boeing H. The MSM program: web-based statistics package for estimating usual dietary intake using the Multiple Source Method. Nature. 2011;87-91.

Mccrory MA, Hajduk CL, Roberts SB. Procedures for screening out inaccurate reports of dietary energy intake. Public Health Nutr [Internet]. 2002;5(6a):873-82. Available from: http://www.journals.cambridge.org/abstract_S1368980002001167

Vinken AG, Bathalon GP, Sawaya AL, Dallal GE, Tucker KL, Roberts SB. Equations for predicting the energy requirements of healthy adults aged 18–81 y 1–3. Am J Clin Nutr. 1999;920-6.

Westerterp-Plantenga MS, Lemmens SG, Westerterp KR. Dietary protein - Its role in satiety, energetics, weight loss and health. Br J Nutr. 2012;108(SUPPL. 2).

Cermak NM, Res PT, Groot LC De, Saris WHM, Loon LJC Van. Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr. 2012;1454-64.

Da Silva-Grigoletto ME, Viana-Montaner BH, Heredia JR, Mata F, Peña G, Brito CJ, et al. Validación de la escala de valoración subjetiva del esfuerzo OMNI-GSE para el control de la intensidad global en sesiones de objetivos múltiples en personas mayores. Kronos - La Rev científica Act física y Deport. 2013;12(1):32-40.

Sanyal A, Naumann J, Hoffmann LS, Chabowska-Kita A, Ehrlund A, Schlitzer A, et al. Interplay between Obesity-Induced Inflammation and cGMP Signaling in White Adipose Tissue. Cell Rep. 2017;18(1):225-36.

Chen L, Chen R, Wang H, Liang F. Mechanisms Linking Inflammation to Insulin Resistance. Int J Endocrinol. 2015; 508409. doi: 10.1155/2015/508409.

Pauli JR, Cintra DE, Souza CT de, Ropelle ER. Novos mecanismos pelos quais o exercício físico melhora a resistência à insulina no músculo esquelético. Arq Bras Endocrinol Metabol. 2009;53(4):399-408.

Dalle S, Rossmeislova L, Koppo K. The role of inflammation in age-related sarcopenia. Front Physiol. 2017;8(DEC).

Franceschi C, Campisi J. Chronic inflammation (Inflammaging) and its potential contribution to age-associated diseases. Journals Gerontol - Ser A Biol Sci Med Sci. 2014;69:S4-9.

Published
2022-06-23
How to Cite
Almeida, J. A., Pires, L. V., Lima, . L. E., Santana, F. B., Júnior, W. M. da S., Grigoletto, M. E. da S., & Netto, R. S. M. (2022). Postmenopausal women with high TNF-α concentrations presented less reduction in fat and blood lipids: A randomized controlled clinical trial. PAJAR - Pan American Journal of Aging Research, 10(1), e42759. https://doi.org/10.15448/2357-9641.2022.1.42759