Efeito da dexametasona e do meloxicam sobre o extravasamento plasmático induzido por carragenina na ATM de ratos
Abstract
A dor orofacial comumente ocorre devido à inflamação aguda ou crônica. Porém, pouco se sabe sobre os mecanismos fisiopatológicos envolvidos na inflamação e na dor inflamatória presentes nas disfunções temporomandibulares. Nosso objetivo foi desenvolver um modelo para o estudo da inflamação aguda na região da articulação temporomandibular (ATM) de ratos utilizando carragenina (CA) e verificar os possíveis efeitos de drogas antiinflamatórias nesse modelo. A inflamação foi avaliada através do extravasamento plasmático (EP) do corante azul de Evans, por espectrofotometria comparada à ATM contralateral que serviu como controle e recebeu injeção de salina. Um experimento com relação ao tempo do efeito da CA sobre o EP do corante Azul de Evans revelou um efeito máximo no tempo de 60 min após a administração. O experimento dose resposta demonstrou que a administração de CA a partir da dose de 300 μg/50 μL, causava um EP estatisticamente significante em relação ao controle. A administração de drogas antiinflamatórias (dexametasona e meloxicam) somente foram capazes de reduzir a inflamação em altas doses. Concluímos que pico de EP induzido pela administração periarticular de CA ocorre em 60 minutos e que o EP induzido pela CA pode ser inibido pelos antiinflamatórios dexametasona e meloxicam.
UNITERMOS: disfunção temporomandibular; inflamação; carragenina; extravasamento plasmático; dexametasona; meloxicam.
SUMMARY
Orofacial pain is most comonly due to acute or chronic inflammation. But, little is known about the mechanism(s) involved in both initiation and maintenance of inflammation and pain related to temporomandibular dysfunction. Our objective was to develop an orofacial model of acute inflammation in rats, using carrageenan and verify the possible effects of antiinflamatory drugs in this model. The inflammation was evalued by protein plasma extravasation of blue Evans’ dye through spectrophotometry compared to contralateral TMJ that was the control. An experiment related to the time of the carrageenan effect on plasma extravasation of blue Evans´ dye showed a maximal effect at 60 min after carrageenan administration. The dose-response experiment showed that from a dose of 300 μg/50 μl, there was a plasma extravasation statiscally significant compared to the control. The administration of antiinflamatory drugs (dexamethasone and meloxican) only were able to reduce the inflammation in high doses. In conclusion: 1) the intra-articular administration of carrageenan in rats can induce a quantified plasma extravasation in 60 minutes; 2) the pre-treatment with meloxicam and dexamethasone minimizes the plasma extravasation induced by carrageenan.
UNITERMS: temporomandibular dysfunction; inflammation; carrageenan; plasma extravasation; dexamethasone; meloxicam.
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