Influence of polymorphisms in osteoprotegerin on susceptibility to periodontal disease in patients with type 2 diabetes

  • Keila Cristina Raposo Lucena
  • Mariana Perez Corrêa
  • Jair Carneiro Leão
  • Paulo Roberto Eleutério de Souza
  • Renata Cimões Federal University of Pernambuco
  • Alessandra de Albuquerque Tavares Carvalho
Keywords: Diabetes, periodontitis, osteoprotegerin, polymorphism,

Abstract

Objective: The aim of this study was to investigate polymorphisms in the promoter region (-163A/G, -245T/G and -950T/C) of the gene osteoprotegerin (OPG) as well as the distribution and influence of these polymorphisms in patients with type 2 diabetes and periodontitis in comparison to a health control group. Methods: The study involved the participation of 67 subjects: 32 in the test group (diabetic patients with periodontitis) and 35 in the control group (non-diabetic subjects without periodontitis). Pocket depth, bleeding on probing and attachment loss were assessed for the diagnosis of periodontitis. Six sites on each tooth were probed. Periodontitis was diagnosed when two or more sites had a pocket depth and attachment loss ≥ 4 mm. DNA was obtained from the blood of the subjects for the investigation of OPG polymorphisms using the Restriction Fragment Length Polymorphism (RFLP) method. Results: No association was found between periodontitis and polymorphisms in the promoter region of the OPG gene in test group (P>0.005). The most frequent allele in this group was A163 (81.2%), followed by T245 (75.0%) and T950 (54.7%). The T950 allele, which is a possible marker of osteoclastogenesis, was not associated with periodontal status in patients with diabetes (P>0.005). Conclusion: No association was found between periodontiitis and genetic polymorphisms in the OPG gene in patients with diabetes.

References

Duarte PM, Neto JBG, Casati MZ, Sallum EA et al. Diabetes modulates gene expression in the gengival tissues of patients with chronic periodontitis. Oral Diseases 2007; 13:594-9.

Novak MJ, Potter RM, Blodgett J, Ebersole JL. Periodontal disease in Hispanic American with type 2 diabetes. J Periodontol 2008; 79:629-36.

Salvi GE, Carollo-Bittel B, Lang NP. Effects of diabetes mellitus on

periodontal and Peri-implant conditions: update on associations and risks. J Clin Periodontol 2008; 35 Suppl 8: 398-409.

Andersen CCP, Flyvbjerg A, Buschard K, Holmstrup P. Relationship

between periodontitis and diabetes: lessons from rodents studies. J

Periodontol 2007; 78(7):1264-75.

Costa PP, Trevisan GL, Macedo GO, Palioto DB et al. Salivary interleukin-6, matrix metalloproteinase-8 and osteoprotegerin in patients with periodontitis and diabetes. J Periodontol 2010; 81(3):384-91.

Frodge BD, Ebersole JL, Kryscio RJ, Thomas MV et al. Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol 2008; 79: 1913-1919.

Lappin DF, Eapen B, Robertson D, Young J, et al. Markers of bone

destruction and formation and periodontitis in type 1 diabetes mellitus. J Clin Periodontol 2009; 36:634-41.

Boyce BF, Xing L. Biology of RANK, RANKL and osteoprotegerin. Arthritis Research & Therapy 2007; 9 Suppl 1:1-7.

Wagner J, Kaminski WE, Aslanidis C, Moder D et al. Prevalence of OPG and IL-1 gene polymorphismin chronic periodontitis. J Clin Periodontol 2007; 34:823-7.

Ribeiro FV, Mendonça AC, Santos VR, Bastos MF et al. Cytokines

and bone-related factors in systemically healthy patients with chronic periodontitis and patients with type 2 diabetes and chronic periodontitis. J Periodontol 2011; 82:1187-96.

Cochran DL. Inflammation and bone loss in periodontal disease. J

Periodontol 2008; 79 suppl 8:1569-76.

Taubman MA, Kawai T, Han X. The new concept of periodontal disease pathogenesis requires new and novel therapeutic strategies. J Clin Periodontol 2007; 34:367-9.

Belibasakis NG, Bostanci N, Hashim A, Johansson A et al. Regulation of RANKL and OPG gene expression: in human gingival fibroblast and periodontal ligament cells by Porphyromonas gingivalis: a putative role of the Arg-gingipains. Microbiol Pathogenesis 2007; 43:46-53.

Lappin DF, Sherrabeh S, Jenkins WMM, Macpherson LMD. Effect of

smoking on serum RANKL and OPG in sex, age and clinically matched supportive therapy periodontitis patients. J Clin Periodontol 2007; 34: 271-7.

Duarte PM, Miranda TS, Lima JA, Gonçalves TED et al. Expression of immune-inflamatory markers in sites of chronic periodontitis in type 2 diabetes subjects. J Periodontol 2011. In press.

Langdahl BL, Carstens M, Stenkjaer L, Criksen EF. Polymorphisms in the osteoprotegerin gene are associated with osteoporotic fractures. J Bone Miner Res 2002; 17:1245-55.

Park O-J, Shin S-Y, Choi Y, Kim M-H. et al. The association of osteoprotegerin gene polymorphisms with periodontitis. Oral Diseases 2008; 14: 440-4.

Baioni CS, Souza CM, Ribeiro APB, Luczyszyn SM et al. Analysis of

the association of polymorphism in the osteoprotegerin gene with

susceptibility to chronic kidney disease and periodontitis. J Periodont Res 2008; 43:578-84.

Soedarsono N, Rabello D, Kamei H Fuma D et al. Evaluation of RANK/RANKL/OPG gene polymorphisms in aggressive periodontitis. J Periodontol Res 2006; 41:397-404.

Wohlfahrt JC, Wu T, Hodges JS, Hinrichs JE, et al. No association between selected candidate gene polymorphisms and severe chronic periodontitis. J Periodontol 2006; 77:426-36.

O’Sullivan EP, Ashley DT, Davenport C, Devlin N, et al. Osteoprotegerin and biomarkers of vascular inflammation in type 2 diabetes. Diabetes Metab Res Rev 2010; 26:496-502.

Araújo NC, Bello DMA, Crovella S, Souza PRE, Donos N, Cimoes R.

Manose binding lectine genes (MBL2) polymorphisms and the periodontal disease in diabetic patients. Rev Odonto Cienc 26(3):203-8.

Vidal C, Brincat M, Xuereb AA. TNFRSF11B gene variants and bone

mineral density in postmenopausal women in Malta. Maturitas 2006; 53:386-95.

Brandstrom H, Stiger F, Lind L, Kahan T, et al. A single nucleotide

polymorphism in the promoter region of the human gene for

osteoprotegerin is related to vascular morphology and function. Biochem Biophys Res Commun 2002; 293:13-7.

Solfi M, Schoppet M, Sattler AM et al. Osteoprotegerin gene polymorphisms in men with coronary artery disease. J Clin Endocrinol Metab 2004; 89:3764-8.

Published
2013-02-04
Section
Original Article