Influence of polymorphisms in osteoprotegerin on susceptibility to periodontal disease in patients with type 2 diabetes

  • Keila Cristina Raposo Lucena
  • Mariana Perez Corrêa
  • Jair Carneiro Leão
  • Paulo Roberto Eleutério de Souza
  • Renata Cimões Federal University of Pernambuco
  • Alessandra de Albuquerque Tavares Carvalho
Keywords: Diabetes, periodontitis, osteoprotegerin, polymorphism,


Objective: The aim of this study was to investigate polymorphisms in the promoter region (-163A/G, -245T/G and -950T/C) of the gene osteoprotegerin (OPG) as well as the distribution and influence of these polymorphisms in patients with type 2 diabetes and periodontitis in comparison to a health control group. Methods: The study involved the participation of 67 subjects: 32 in the test group (diabetic patients with periodontitis) and 35 in the control group (non-diabetic subjects without periodontitis). Pocket depth, bleeding on probing and attachment loss were assessed for the diagnosis of periodontitis. Six sites on each tooth were probed. Periodontitis was diagnosed when two or more sites had a pocket depth and attachment loss ≥ 4 mm. DNA was obtained from the blood of the subjects for the investigation of OPG polymorphisms using the Restriction Fragment Length Polymorphism (RFLP) method. Results: No association was found between periodontitis and polymorphisms in the promoter region of the OPG gene in test group (P>0.005). The most frequent allele in this group was A163 (81.2%), followed by T245 (75.0%) and T950 (54.7%). The T950 allele, which is a possible marker of osteoclastogenesis, was not associated with periodontal status in patients with diabetes (P>0.005). Conclusion: No association was found between periodontiitis and genetic polymorphisms in the OPG gene in patients with diabetes.


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Original Article