Influence of polymorphisms in osteoprotegerin on susceptibility to periodontal disease in patients with type 2 diabetes
Keywords:
Diabetes, periodontitis, osteoprotegerin, polymorphism,Abstract
Objective: The aim of this study was to investigate polymorphisms in the promoter region (-163A/G, -245T/G and -950T/C) of the gene osteoprotegerin (OPG) as well as the distribution and influence of these polymorphisms in patients with type 2 diabetes and periodontitis in comparison to a health control group. Methods: The study involved the participation of 67 subjects: 32 in the test group (diabetic patients with periodontitis) and 35 in the control group (non-diabetic subjects without periodontitis). Pocket depth, bleeding on probing and attachment loss were assessed for the diagnosis of periodontitis. Six sites on each tooth were probed. Periodontitis was diagnosed when two or more sites had a pocket depth and attachment loss ≥ 4 mm. DNA was obtained from the blood of the subjects for the investigation of OPG polymorphisms using the Restriction Fragment Length Polymorphism (RFLP) method. Results: No association was found between periodontitis and polymorphisms in the promoter region of the OPG gene in test group (P>0.005). The most frequent allele in this group was A163 (81.2%), followed by T245 (75.0%) and T950 (54.7%). The T950 allele, which is a possible marker of osteoclastogenesis, was not associated with periodontal status in patients with diabetes (P>0.005). Conclusion: No association was found between periodontiitis and genetic polymorphisms in the OPG gene in patients with diabetes.References
Duarte PM, Neto JBG, Casati MZ, Sallum EA et al. Diabetes modulates gene expression in the gengival tissues of patients with chronic periodontitis. Oral Diseases 2007; 13:594-9.
Novak MJ, Potter RM, Blodgett J, Ebersole JL. Periodontal disease in Hispanic American with type 2 diabetes. J Periodontol 2008; 79:629-36.
Salvi GE, Carollo-Bittel B, Lang NP. Effects of diabetes mellitus on
periodontal and Peri-implant conditions: update on associations and risks. J Clin Periodontol 2008; 35 Suppl 8: 398-409.
Andersen CCP, Flyvbjerg A, Buschard K, Holmstrup P. Relationship
between periodontitis and diabetes: lessons from rodents studies. J
Periodontol 2007; 78(7):1264-75.
Costa PP, Trevisan GL, Macedo GO, Palioto DB et al. Salivary interleukin-6, matrix metalloproteinase-8 and osteoprotegerin in patients with periodontitis and diabetes. J Periodontol 2010; 81(3):384-91.
Frodge BD, Ebersole JL, Kryscio RJ, Thomas MV et al. Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol 2008; 79: 1913-1919.
Lappin DF, Eapen B, Robertson D, Young J, et al. Markers of bone
destruction and formation and periodontitis in type 1 diabetes mellitus. J Clin Periodontol 2009; 36:634-41.
Boyce BF, Xing L. Biology of RANK, RANKL and osteoprotegerin. Arthritis Research & Therapy 2007; 9 Suppl 1:1-7.
Wagner J, Kaminski WE, Aslanidis C, Moder D et al. Prevalence of OPG and IL-1 gene polymorphismin chronic periodontitis. J Clin Periodontol 2007; 34:823-7.
Ribeiro FV, Mendonça AC, Santos VR, Bastos MF et al. Cytokines
and bone-related factors in systemically healthy patients with chronic periodontitis and patients with type 2 diabetes and chronic periodontitis. J Periodontol 2011; 82:1187-96.
Cochran DL. Inflammation and bone loss in periodontal disease. J
Periodontol 2008; 79 suppl 8:1569-76.
Taubman MA, Kawai T, Han X. The new concept of periodontal disease pathogenesis requires new and novel therapeutic strategies. J Clin Periodontol 2007; 34:367-9.
Belibasakis NG, Bostanci N, Hashim A, Johansson A et al. Regulation of RANKL and OPG gene expression: in human gingival fibroblast and periodontal ligament cells by Porphyromonas gingivalis: a putative role of the Arg-gingipains. Microbiol Pathogenesis 2007; 43:46-53.
Lappin DF, Sherrabeh S, Jenkins WMM, Macpherson LMD. Effect of
smoking on serum RANKL and OPG in sex, age and clinically matched supportive therapy periodontitis patients. J Clin Periodontol 2007; 34: 271-7.
Duarte PM, Miranda TS, Lima JA, Gonçalves TED et al. Expression of immune-inflamatory markers in sites of chronic periodontitis in type 2 diabetes subjects. J Periodontol 2011. In press.
Langdahl BL, Carstens M, Stenkjaer L, Criksen EF. Polymorphisms in the osteoprotegerin gene are associated with osteoporotic fractures. J Bone Miner Res 2002; 17:1245-55.
Park O-J, Shin S-Y, Choi Y, Kim M-H. et al. The association of osteoprotegerin gene polymorphisms with periodontitis. Oral Diseases 2008; 14: 440-4.
Baioni CS, Souza CM, Ribeiro APB, Luczyszyn SM et al. Analysis of
the association of polymorphism in the osteoprotegerin gene with
susceptibility to chronic kidney disease and periodontitis. J Periodont Res 2008; 43:578-84.
Soedarsono N, Rabello D, Kamei H Fuma D et al. Evaluation of RANK/RANKL/OPG gene polymorphisms in aggressive periodontitis. J Periodontol Res 2006; 41:397-404.
Wohlfahrt JC, Wu T, Hodges JS, Hinrichs JE, et al. No association between selected candidate gene polymorphisms and severe chronic periodontitis. J Periodontol 2006; 77:426-36.
O’Sullivan EP, Ashley DT, Davenport C, Devlin N, et al. Osteoprotegerin and biomarkers of vascular inflammation in type 2 diabetes. Diabetes Metab Res Rev 2010; 26:496-502.
Araújo NC, Bello DMA, Crovella S, Souza PRE, Donos N, Cimoes R.
Manose binding lectine genes (MBL2) polymorphisms and the periodontal disease in diabetic patients. Rev Odonto Cienc 26(3):203-8.
Vidal C, Brincat M, Xuereb AA. TNFRSF11B gene variants and bone
mineral density in postmenopausal women in Malta. Maturitas 2006; 53:386-95.
Brandstrom H, Stiger F, Lind L, Kahan T, et al. A single nucleotide
polymorphism in the promoter region of the human gene for
osteoprotegerin is related to vascular morphology and function. Biochem Biophys Res Commun 2002; 293:13-7.
Solfi M, Schoppet M, Sattler AM et al. Osteoprotegerin gene polymorphisms in men with coronary artery disease. J Clin Endocrinol Metab 2004; 89:3764-8.
Downloads
Published
Issue
Section
License
COPYRIGHT
The submission of originals to Odonto Ciência implies the transfer by the authors of the right for publication. Authors retain copyright and grant the journal right of first publication. If the authors wish to include the same data into another publication, they must cite Odonto Ciência as the site of original publication.
CREATIVE COMMONS LICENSE
As this journal is open access, the articles are allowed free use in scientific and educational applications, with citation of the source.
According to the type of Creative Commons License (CC-BY 4.0) adopted by Odonto Ciência, the user must respect the requirements below.
You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
However, only under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests Odonto Ciência endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
For more details on the Creative Commons license, please follow the link in the footer of this website.
