Weight gain and metabolic changes in mice submitted to high fat diet
DOI:
https://doi.org/10.15448/1983-652X.2018.1.27672Keywords:
models animal, obesity, glucose metabolism disorders, lipid metabolism disorders.Abstract
Objective: The aim of this study was to evaluate the efficiency of high fat diet in the induction of obesity and glucose and lipid metabolism disorders in C57BL/6 mice.
Materials and Methods: Animals were organized into two groups, totalizing 19 C57BL/6 mice: control group (commercial diet; n=9) and experimental group (high fat diet; n=10). Food intake and weight gain were measured during 12 weeks and Food Efficiency Coefficient (FEC) and Aterogenic Index were calculated. The Oral Glucose Tolerance Test (TOTG) was performed, the glycemic curve was constructed and the area under the curve (AUC) was verified. Liver and adipose tissue (AT) sites were dissected and histological analysis of the liver and epididymal adipose tissue were performed. The Adiposity Index (AI%) was calculated and the concentration of total cholesterol, its fractions, glucose and serum triglycerides were measured.
Results: The experimental group presented lower food intake (p<0.001) and higher total weight gain (p=0.001) and FEC (p<0.001). It was observed in the experimental group an increase in the epididymal (p<0.001), retroperitoneal (p<0.001), mesenteric (p=0.002), perirenal AT (p=0.012), total fat (p <0.001) and AI% (p<0.001). Total cholesterol was higher in the experimental group (p=0.025) and the HDL lower in the control group (p=0.005). The adipocyte area of the experimental group was also higher than the control group (p=0.049). In the other parameters there was no significant difference between groups.
Conclusion: This model induced obesity in a short period of time and promoted increased visceral adiposity with a low-cost diet. Further studies are needed to improve its efficiency and to completely change blood lipids and glycemia in C57BL/6 mice.
References
Galic S, Oakhill JS, Steinberg GR. Adipose tissue as an endocrine organ. Mol Cell Endocrinol. 2010;316(2):129-39. https://doi.org/10.1016/j.mce.2009.08.018
Lee S, Bacha F, Silva AA. Waist circumference, blood pressure, and lipid components of the metabolic syndrome. J Pediatr. 2006;149(6):809-16. https://doi.org/10.1016/j.jpeds.2006.08.075
Sant'Anna S, Caiado NM, Silva LJ. Neuromodulação hipotalâmica: uma proposta terapêutica para obesidade. Rev Med Saude Brasília. 2014;3(2):192-204.
Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância de Doenças e Agravos não Transmissíveis e Promoção da Saúde. Vigitel Brasil 2015: estimativas sobre frequência e distribuição sociodemográfica de fatores de risco e proteção para doenças crônicas nas capitais dos 26 estados brasileiros e no Distrito Federal em 2015. Brasília: Ministério da Saúde; 2016.
Soares FLP. Efeitos e mecanismos de ação da exclusão dietética de glúten de trigo na obesidade e resistência à insulina experimentais [tese]. Minas Gerais: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas; 2012.
Mosca PRF, Silveira PP, Werlang ICR, Goldani MZ. Obesidade e genética. Rev HCPA. 2012;32(3):318-31.
Reeves PG, Nielsen FH, Fahey GC JR. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123(11):1939-51. https://doi.org/10.1093/jn/123.11.1939
Rosini TC, Silva ASR, Moraes C. Obesidade induzida por consumo de dieta: modelo em roedores para o estudo de distúrbios relacionados com a obesidade. Rev Assoc Med Bras. 2012;58(3):383-7. https://doi.org/10.1590/S0104-42302012000300021
Soares FLP, Santana L, Pinto LA, Dutra TS, Freitas KC, Kassuya CAL. Novo modelo experimental de síndrome metabólica induzida por dieta. In: 12º Congresso da Sociedade Brasileira de Alimentação e Nutrição; 2013; Foz do Iguaçu. São Paulo: Nutrire - Sociedade Brasileira de Alimentação e Nutrição - SBAN; 2013. p. 234-4.
Chu HF, Pan MH, Ho CT, Tseng YH, Wang WW, Chau CF. Variations in the Efficacy of Resistant Maltodextrin on Body Fat Reduction in Rats Fed Different High-Fat Models. J Agric Food Chem. 2014;62(1): 192-7. https://doi.org/10.1021/jf404809v
Moreira AS. Dietas ricas em gordura, frutose e sacarose: alterações no fígado [tese]. Rio de Janeiro: Universidade Estadual do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes; 2014.
Freitas KC, Amancio OMS, Morais MB. High-performance inulin and oligofructose prebiotics increase the intestinal absorption of iron in rats with iron deficiency anaemia during the growth phase. Br J Nutr. 2012;108(6):1008-16. https://doi.org/10.1017/S0007114511006301
Machado H, Pinheiro HS, Terra MM, Guerra MO, de Paula RB, Peters VM. Dissociação da resposta anti-hipertensiva e metabólica à losartana e espironolactona em ratos com síndrome metabólica experimental. J Bras Nefrol. 2012;34(4):328-36. https://doi.org/10.5935/0101-2800.20120021
Taylor BA, Phillips SJ. Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling. Genomics. 1996;34(302):389-98. https://doi.org/10.1006/geno.1996.0302
Mendonça FS, Evêncio-Neto J, Simões MJ, Simões RS, Baratella Evêncio L. Freqüência de hipoplasia ovariana em vacas abatidas em Rondônia. Arq Inst Biol. 2006;68(Supl):267-70.
Aguila MB, Pinheiro AR, Parente LB, Mandarim-de-Lacerda CA. Dietary effect of different high-fat diet on rat liver stereology. Liver Int. 2003;23:363-70. https://doi.org/10.1034/j.1478-3231. 2003.00858.x
Catta-Preta M, Mendonça LS, Fraulob-Aquino J, Aguila MB, Mandarim-de-Lacerda CA. A critical analysis of three quantitative methods of assesment of hepatic steatosis in liver biopsies. Virchows Arch. 2011;459:477-85. https://doi.org/10.1007/s00428-011-1147-1
Florence NT, Benoit MZ, Jonas K, Alexandra T, Désiré DDP, Pierre K, Théophile D. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. J Ethnopharmacol. 2014;151(2):784–90. https://doi.org/10.1016/j.jep.2013.09.021
Carvalho KMB, Dutra ES. Obesidade. In: Cuppari L. Nutrição clínica no adulto. 3ª ed. Barueri, SP: Manole; 2014. p. 185-214.
Ferreira LM, Hochman B, Barbosa MVJ. Modelos experimentais em pesquisa. Acta Cir Bras. 2005;20(2):28-34. https://doi.org/10.1590/S0102-86502005000800008
Avesani CM, Santos NSJ, Cuppari L. In: Cuppari L. Nutrição Clínica no Adulto. 3ª ed. Barueri, SP: Manole; 2014. p. 45-62.
Buettner R, Schölmerich J, Bollheimer LC. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity. 2007;15(4):798–808. https://doi.org/10.1038/oby.2007.608
White PAS, Cercato LM, Araújo JMD, Souza LA, Soares AF, Barbosa APO, R Neto JM, Marçal AC, Machado UF, Camargo EA, Santos MRV, Brito LC. Modelo de obesidade induzida por dieta hiperlipídica e associada à resistência à ação da insulina e intolerância à glicose. Arq Bras Endocrinol Metab. 2013;57(5):339-45. https://doi.org/10.1590/S0004-27302013000500002
Barroso SG, de Abreu VG, Francischetti EA. A Participação do tecido adiposo visceral na gênese da hipertensão e doença cardiovascular aterogênica. Arq Bras Cardiol. 2002;78(6):618-30. https://doi.org/10.1590/S0066-782X2002000600012
Kiperstok AC. Avaliação longitudinal da função cardíaca em modelo experimental de diabetes tipo 2 induzido por dieta hipergordurosa em camundongo [dissertação]. Salvador: Fundação Oswaldo Cruz; 2010.
Ravagnani FCP, Ravagnani CFC, Braga Neto JA, Voltarelli FA, Zavala AAZ, Habitante CA, Inouye CM. Efeito de dietas hiperlipídicas com extrato de baru e chocolate sobre a área de adipócitos de ratos submetidos ao exercício físico. Rev Bras Med Esporte. 2012;18(3):190-4. https://doi.org/10.1590/S1517-86922012000300011
Gutierrez DA, Puglisi MJ, Hasty AH. Impact of increased adipose tissue mass on inflammation, insulin resistance, and dyslipidemia. Curr Diab Rep. 2009;9(1):26-32. https://doi.org/10.1007/s11892-009-0006-9
Martínez G, Alonso R, Novik V. Síndrome metabólico: bases clínicas y fisiopatológicas para un enfoque terapéutico racional. Rev Méd Chile. 2009;137(5):685-94. https://doi.org/10.4067/S0034-98872009000500014
Fonseca FAH. Síndrome metabólica sem obesidade central: mito ou realidade? Rev Bras Hipertens. 2008;15(3):168-9.
Almeida MEF, Medeiros RS, Figueiredo FJB, Coelho EJB, Sena MPT. Efeitos do estresse auditivo e da dieta hipercalórica sobre o peso corporal, lipídios e glicemia de ratos wistar. Alim Nutr. 2011;22(3):359-65.
Santos RD, Gagliardi ACM, Xavier HT, Magnoni CD, Cassani R, Lottenberg AMP,Casella Filho A, Araújo DB, Cesena FY, Alves RJ, Fenelon G, Nishioka SAD, Faludi AA, Geloneze B, Scherr C, Kovacs C, Tomazzela C, Carla C, Barrera-Arellano D, Cintra D, Quintão E, Nakandakare ER, Fonseca FAH, Pimentel I, Santos JE, Bertolami MC, Rogero M, Izar MC, Nakasato M, Damasceno NRT, Maranhão R, Cassani RSL, Perim R, Ramos S; Sociedade Brasileira de Cardiologia. I Diretriz sobre o consumo de gorduras e saúde cardiovascular. Arq Bras Cardiol. 2013;100(1 Supl. 3):1-40. https://doi.org/10.5935/abc.2013S003
Almeida e Borges VF, Diniz ALD, Cotrim HP, Rocha HLOG, Salomão FC. Dopplerfluxometria da veia hepática em pacientes com esteatose não alcoólica. Radiol Bras. 2011;44(1):1-6. https://doi.org/10.1590/S0100-39842011000100005
Takahashi Y, Soejima Y, Fukusato T. Animal models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis. World J Gastroenterol. 2012;18(19):2300-8. https://doi.org/10.3748/wjg.v18.i19.2300
Carvalheira JBC, Saad MJA. Doenças associadas à resistência à insulina/ hiperinsulinemia, não incluídas na síndrome metabólica. Arq Bras Endocrinol Metab. 2006;50(2):360-7. https://doi.org/10.1590/S0004-27302006000200022
Sociedade Brasileira de Diabetes. Diretrizes da Sociedade Brasileira de Diabetes: 2013-2014. São Paulo: AC Farmacêutica; 2014.
Chiarelli F, Marcovecchio ML. Insulin resistance and obesity in childhood. Eur J Endocrinol. 2008;159(1):67-74. https://doi.org/10.1530/EJE-08-0245
Davies MJ, Raymond NT, Day JL, Hales CN, Burden AC. Impaired glucose tolerance and fasting hyperglycaemia have different characteristics. Diabet Med. 2000;17(6):433-40. https://doi.org/10.1046/j.1464-5491.2000.00246.x
Ferreira PA, Salame JP, Bastos RF, Francisco LS, Felix AOC, Felix SR, Nobre MO. Considerações sobre a dieta ain-93m elaborada para modelos biológicos. Resbcal. 2015;3(2):103-8.
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