Molecular characterization of Streptococcus mutans gtfB gene isolated from families

Authors

  • Monica Moreira Post Graduated Program in Engineer of Bioprocess and Biotechnology of Federal University of Paraná – UFPR - Brazil
  • Mariana Machado Fidelis do Nascimento Post Graduated Program in Microbiology, Parasitology e Pathology of Federal University of Paraná – UFPR – Brazil
  • Mariane Moreira Poletto Post Graduated Program in Genetics of Federal University of Paraná – UFPR – Brazil
  • Débora do Rocio Kliisiowicz Post Graduated Program in Microbiology, Parasitology e Pathology of Federal University of Paraná – UFPR – Brazil
  • Renata Rodrigues Gomes Post Graduated Program in Microbiology, Parasitology e Pathology of Federal University of Paraná – UFPR – Brazil
  • Vânia Aparecida Vicente Post Graduated Program in Engineer of Bioprocess and Biotechnology of Federal University of Paraná – UFPR - Brazil Post Graduated Program in Microbiology, Parasitology e Pathology of Federal University of Paraná – UFPR – Brazil Fellowship from Conselho Nacional de Pesquisa e Desenvolvimento - CNPq

DOI:

https://doi.org/10.15448/1980-6523.2018.1.29727

Keywords:

Streptococcus mutans, glucosyltransferase β, virulence.

Abstract

OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.
METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.
RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.
CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.

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Published

2018-12-30

Issue

Vol. 33 No. 1 (2018)

Section

Original Article

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