In vitro evaluation of the effect of vinegar solutions on the microhardness of root dentin
DOI:
https://doi.org/10.15448/1980-6523.2014.1.11504Keywords:
Acid acetic, dentin, EDTA, hardness test, root canal irrigants,Abstract
Objective: This in vitro study evaluated the action of vinegar solutions on microhardness of root dentin. Methods: Ten extracted human maxillary central incisors were sectioned transversely at the cementenamel junction. The roots were fixed on cutting machine device and were cut on cervical region transversely in 2-mm sections. The second cervical section was divided into four parts. Each one quarter of section was fixed with wax in the center of a pre-fabricated acrylic disc. Four treatment groups were tested: group 1 – alcohol vinegar; group 2 – white wine vinegar; group 3 – EDTA (standard group); group 4 – distilled water (control group). The specimens were submitted to solutions application for 5 min. The measurements of microhardness (initial and post-treatment) were carried out using a Knoop microhardness tester. Data were analyzed by using Wilcoxon and Kruskal Wallis tests. Results: The analysis of initial and final microhardness showed statically significant differences before and after application of the tested solutions alcohol vinegar, white wine vinegar and EDTA. Groups 1,2 and 3 presented a decrease in the microhardness values. Conclusion: The solutions of alcohol vinegar and white wine vinegar, as well as the EDTA, reduced the microhardness of root dentin.References
McComb D, Smith DC. A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod 1975;1: 238-42.
Kokkas AB, Boutsioukis AC, Vassiliadis LP, Stavrianos CK. The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: an in vitro study. J Endod 2004;30:100-2.
Torabinejad M, Handysides R, Khademi AA, Bakland LK. Clinical implications of the smear layer in endodontics: A review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;94:658-66.
Prado M, Gusman H, Gomes BP, Simão RA. Scanning electron microscopic investigation of the effectiveness of phosphoric acid in smear layer removal when compared with EDTA and citric acid. J Endod 2011;37:255-8.
Mortazavi V, Khademi A, Khosravi K, Fathi M, Ebrahimi-Chaharom M, Shahnaseri S, et al. Effect of MTAD on the shear bond strength of self-etch adhesives to dentin. Dent Res J 2012;9:24-30.
Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
Dogan H, Çalt S. Effects of chelating agents and sodium hypochlorite on mineral content of root dentin. J Endod 2001;27:578-80.
Fuentes V, Ceballos L, Osorio R, Toledano M, Carvalho RM, Pashley DH. Tensile strength and microhardness of treated human dentin. Dent Mat 2004;20:522-9.
Sayin TC, Serper A, Cehreli ZC, Otlu HG. The effect of EDTA, EGTA, EDTAC, and tetracydcline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med
Oral Pathol Oral Radiol Endod 2007;104:418-24.
Cruz-Filho AM, Sousa-Neto MD, Savioli RN, Silva RG, Vansan LP, Pécora JD. Effect of Chelating Solutions on the Microhardness of Root Canal Lumen Dentin. J Endod 2011;37:358-62.
Marshall GW, Yücel N, Balooch M, Kinney JH, Habelitz S, Marshall SJ. Sodium hypochlorite alterations of dentin and dentin collagen. Surface Science 2001;491:444-55.
Panighi M, G’Sell C. Effect of the tooth microstructure on the shear bond strength of a dental composite. Journal of Biomedical Materials Research 1993;27:975-81.
Yoshiyama M, Tay FR, Doi J, Nishitani Y, Yamada T, Itou K, CarvalhRM, Nakajima M, Pashley DH. Bonding of self-etch and total-etch adhesives to carious dentin. Journal of Dental Research 2002;81:556-60.
Pinto SC, Batitucci RG, Pinheiro MC, Zandim DL, Spin-Neto R, Sampaio JE. Effect of an acid diet allied to sonic tooth brushing on root dentin permeability: an in vitro study. Braz Dent J 2010;21:390-5.
Zandim DL, Corrêa FO, Sampaio JE, Rossa Júnior C. The influence of vinegars on exposure of dentinal tubules: a SEM evaluation. Braz Oral Res 2004;18:63-8
Lazzari EP. Dental Biochemistry. Philadelphia: Lea e Febiger; 1976.
Spanó JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pécora JD. Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod. 2009;35:727-30.
De-Deus G, Reis C, Fidel S, Fidel R, Paciornik S. Dentine demineralization when subjected to EDTA with or without various wetting agents: a co-site digital optical microscopy study. Int Endod J 2008;41:279-87.
De-Deus G, Paciornik S, Maurício MHP. Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J 2006;39:401-6.
Winchell H. The knoop microhardness tester as a mineralogical tool. Am Mineral 1945;30:583-95.
Lysaght VE, DeBellis A. Microhardness testing. In: Wilkes B. Hardness testing handbook. New York: American Chain and Cable Co.;1969:76-105.
Knoop F, Peters CG, Emersoon WB. A sensitive pyramidal-diamond tool for indentation measurements. J Res Nat Bur Stand 1939;23:39-61.
Souza SA. Ensaios de Dureza. In: Souza AS. Ensaios Mecânicos de Materiais Metálicos: Fundamentos teóricos e Práticos. 5th. São Paulo: Edgard Blücher: 1982;103-36.
Hülsmann M, Heckendorff M, Schafers F. Comparative in-vitro evaluation of three chelator pastes. Int Endod J 2002;35:668-79.
Hulsmann M, Heckendorff M, Lennon Á. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 2003; 36:810-30.
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