Histological evaluation of the osteoinductive potential of demineralized dentin matrix

Authors

  • Manoel Eduardo de Lima Machado University of São Paulo
  • Victor Di Donato Marques
  • Cleber Keiti Nabeshima University of São Paulo

Keywords:

Biocompatible materials, dental implantation, dentin,

Abstract

Objective: The aim of this study was to verify the osteoinductivity of the xenogeneic and ectopic implantation of demineralized dentin matrix (DDM) in muscle tissue of rats in short-term. Methods: Ten human teeth were utilized to obtain the DDM. Surgical access was made by incisions on two pockets created in the dorsal musculature of 10 Wistar rats, where the implants were inserted. The animals were divided into 2 groups: in the first group the implants remained in place for one month and in the second group for two months. Biopsies were taken at the end of the experimental times. Results: Histological examination showed the presence of an intense chronic inflammatory infiltrate, and in some areas, osteoclasts were observed surrounding the material, and no mineralization foci were found. Conclusion: It can be concluded that DDM implanted for periods of 1 or 2 months in the dorsal musculature of rats did not result in osteoinduction.

References

Paulo AO, Castro-Silva II, Oliveira DF, Machado MEL, Bonetti-Filho I, Granjeiro JM. Repair of critical-size defects with autogenous periosteumderived cells combined with bovine anorganic apatite/collagen: An experimental study in rat calvaria. Braz Dent J 2011;22:322-8.

Carvalho VAP, Tosello DO, Salgado MAC, Gomes MF. Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles. Int J Oral Maxillofac Implants 2004;19: 679-86.

Conover MA, Urist MR. Transmembrane bone morphogenesis by implants of dentin matrix. J Dent Res 1979; 58:1911.

Gomes MF, Dos Anjos MJ, Nogueira TO, Catanzaro Guimarães AS. Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies. Int J Oral Maxillofac Implants 2002;17:488-97.

Lissenberg-Thunnissen SN, Gorter DJJ, Sier FM, Schipper IB. Use and efficacy of bone morphogenetic proteins in fracture healing. Int Orthop 2011;35:1271-80.

Casagrande L, Demarco FF, Zhang Z, Araujo FB, Shi S, Nör JE. Dentinderived BMP-2 and odontoblast differentiation. J Dent Res 2010;89:603-8.

Carstens MH, Chin M, Li XJ. In situ osteogenesis: regeneration of 10-cm mandibular defect in porcine model using recombinant human bone morphogenetic protein-2 (rhBMP-2) and helistat absorbable collagen

sponge. J Craniofac Surg 2005;16:1033-42.

Barr T, McNamara AJA, Sándor GKB, Clokie CML. Comparison of the osteoinductivity of bioimplants containaing recombinant human bone morphogenetic proteins 2 (Infuse) and 7 (OP-1). Oral Surg Oral Med Oral

Pathol Oral Radiol Endod 2010;109:531-40.

Katayama Y, Matsuyama Y, Yoshihara H, Sakai Y, Nakamura H, Imagama S, Ito Z, Wakao N, Kamiya M, Yukawa Y, Kanemura T, Sato K, Iwata H, Ishiguro N. Clinical and radiographic outcomes of posterolateral lumbar spine fusion in humans using recombinant human bone morphogenetic protein-2: an average five-years follow-up study. Int Orthop 2009;33: 1061-7.

De Biase P, Capanna R. Clinical application of BMPs. Int J Care Injured 2005;36S(3):S43-6.

Hao J, Zou B, Narayanan K, George A. Differential expression patterns of the dentin matrix proteins during mineralized tissue formation. Bone 2004;34:921-32.

Srinivasan R, Chen B, Gorski JP, George A. Recombinant expression and characterization of dentin matrix protein 1. Connect Tissue Res 1999;40:251-8.

Chaussain C, Eapen AS, Huet E, Floris C, Ravindran S, Hao J, et al. MMP2-cleavage of DMP1 generates a bioactive peptide promoting differentiation of dental pulp steam/progenitor cells. Eur Cell Mater 2009;18:84-95.

Sanada JT, Canova GC, Cestari TM, Taga EM, Buzalaf MAR. Histologic, radiographic and imunoglobuline profile analysis after implantation blocks of demineralized bovine cancellous bone graft in muscle of rats. J Appl

Oral Sci 2003;11:209-15.

Machado MEL, Souza AS, Araújo VC. Histological evaluation of the osteoinduction capability of human dentine. Int Endod J 2006;39: 855-9.

Hofmann A, Konrad L, Hessman MH, Kuchle R, Korner J, Rompe JD, Rommens PM. The influence of bone allograft processing on osteoblast attachment and function. J Orthop Res 2005;23:846-54.

Zhou ZK, Pei FX, Cheng JQ, Tu CQ, Lian YY, Li XQ. Use of cortical bone plates allografts for reconstruction of femoral fracture: an experimental study in goats. Sichuan Da Xue Xue Bao Yi Xue Ban 2005;36:880-4.

Zhang JL, Zheng YY, Hu JA, Chen XP. Effects of surface potentials of tooth hard tissue on bone remodeling in rabbit tibiae. Zhonghua Kou Qiang Yi Xue Za Zhi 2005;40:331-4.

Develioglu H, Saraydin SU, Bolayir G, Dupoiriex L. Assessment of the effect of a biphasic ceramic on bone response in a rat calvarial defect model. J Biomed Mater Res A 2006;77A:627-31.

Economides N, Pantalidou O, Kokkas A, Tziafas D. Short-term periradicular tissue response to mineral trioxide aggregate (MTA) as root-end filling material. Int Endod J 2003;36:44-8.

Jaramilo A, Fernández R, Villa P. Endodontic treatment of dens invaginatusa 5-year follow up. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e15-21.

Dalkys M, Ozcan A, Yapar M, Gokay N, Yuncu M. Evaluation of the effects of different biomaterials on bone defects. Implant Dent 2000;9:226-35.

Leventis MD, Eleftheriadis E, Oikonomopoulou P, Vavouraki H, Khaldi L, Tosios KI, Vardas E, Valavanis KD, Dontas I. Experimental study of the effect of autologous platelet-rich plasma on the early phases of osteoinduction by allogenic demineralized bone matrix. Implant Dent 2012;21:399-405.

Cooper PR, Takahashi Y, Graham LW, Simon S, Imazato S, Smith AJ. Inflammation-regeneration interplay in the dentine-pulp complex. J Dent 2010;38:687-97.

Simon S, Smith AJ, Lumley PJ, Berdal A, Smith G, Finney S, Cooper PR. Molecular characterization of young and mature odontoblasts. Bone 2009;45:693-703.

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Published

2013-02-05

Issue

Vol. 28 No. 1 (2013)

Section

Original Article

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