Effect of two models of stress associated with ligature-induced periodontitis on hematological parameters in rats

Authors

  • Leonardo Stephan Caporossi UNIC
  • Aurélio Rosa da Silva Universidade de Cuiabá -UNIC.
  • Tereza A. Delle Vedove Semenoff UNESP - Araçatuba - SP.
  • Fábio Miranda Pedro Universidade de Cuiabá -UNIC.
  • Alvaro Henrique Borges Universidade de Cuiabá - UNIC, MT.
  • Alex Segundo Semenoff-Segundo CEOPE, UNIC e UNIVAG

Keywords:

Stress, rats, periodontitis

Abstract

Purpose: To evaluate the effect of two models of chronic stress in rats and their association with induced periodontitis on hematological parameters: mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hematocrit (Ht), erythrocytes (Hm), hemoglobin (Hg) and leukocytes (Lk). Methods: Forty-eight adult Wistar rats were randomly distributed into four groups (n=12): physical stress (PSG), variable stress (VSG), ligature (LG) and control (CG) and then started the test of physical stress (restraint and exposure to cold) and variable stress (exposure to flashing light, isolation, examination of the oral cavity, congested environment, the smell of blood and noise). After 10 days of the stress test, the animals in Groups PS, VS and L were anesthetized, and a silk thread was adapted around the upper right second molar; subsequently, the stress test continued for 50 days. The animals were anesthetized and held up the incision and visualization of the posterior vena cava for blood puncture vacuum in tubes containing EDTA. Data were collected by blinded and trained examiners and were statistically analyzed by means of ANOVA and Bonferroni’s test at the significance level of 0.05. Results: The two models of stress changed all of the hematological parameters tested, with the exception of VCM. Conclusion: The stress associated with periodontitis is able to modify blood parameters in rats.

Author Biographies

Leonardo Stephan Caporossi, UNIC

Graduando em Odontologia pela Universidade de Cuiabá -MT.

Aurélio Rosa da Silva, Universidade de Cuiabá -UNIC.

Graduando em Odontologia pela Universidade de Cuiabá -MT.

Tereza A. Delle Vedove Semenoff, UNESP - Araçatuba - SP.

Doutora em Estomatologia pela UNESP - Araçatuba,SP.

Fábio Miranda Pedro, Universidade de Cuiabá -UNIC.

Diretor da Faculdade de Odontologia de Cuiabá - UNIC, MT.

Alvaro Henrique Borges, Universidade de Cuiabá - UNIC, MT.

Professor de Endodontia e Odontologia em âmbito Hospitalar da Universidade de Cuiabá - UNIC,MT.

Alex Segundo Semenoff-Segundo, CEOPE, UNIC e UNIVAG

Centro Estadual de Odontologia para pacientes Especiais - CEOPE, Universidade de Cuiabá -UNIC- e Centro universitário de Várzea Grande - UNIVAG.

Downloads

Published

2010-09-25

Issue

Vol. 25 No. 4 (2010)

Section

Original Article

License

COPYRIGHT
The submission of originals to Odonto Ciência implies the transfer by the authors of the right for publication. Authors retain copyright and grant the journal right of first publication. If the authors wish to include the same data into another publication, they must cite Odonto Ciência as the site of original publication.

CREATIVE COMMONS LICENSE
As this journal is open access, the articles are allowed free use in scientific and educational applications, with citation of the source.
According to the type of Creative Commons License (CC-BY 4.0) adopted by Odonto Ciência, the user must respect the requirements below.

You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material for any purpose, even commercially.

However, only under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests Odonto Ciência endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

For more details on the Creative Commons license, please follow the link in the footer of this website.