Antibacterial action of red and green propolis extract in infected root canal

Authors

  • Mônica Misaé Endo University Center of Anápolis - Unievangélica
  • Cyntia R. A. Estrela University of Cuiabá
  • Ana Helena G. Alencar Federal University of Goiás
  • Júlio Almeida Silva Federal University of Goiás http://orcid.org/0000-0003-0112-5203
  • Daniel Almeida Decurcio Federal University of Endodontics
  • Carlos Estrela Federal University of Goiás

DOI:

https://doi.org/10.15448/1980-6523.2017.2.28930

Keywords:

Endodontic, Propolis, Sodium hypochlorite

Abstract

OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals.
METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols - passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium.
RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective.
CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti inflammatory, antioxidant and low toxicity properties.

Author Biographies

Mônica Misaé Endo, University Center of Anápolis - Unievangélica

Professor of Endodontics, School of Dentistry,
University of Anápolis UniEvangélica, Anápolis,
GO, Brazil

Cyntia R. A. Estrela, University of Cuiabá

Professor of Endodontics, School of Dentistry,
University of Cuiabá, Cuiabá, MT, Brazil

Ana Helena G. Alencar, Federal University of Goiás

Professor of Endodontics, Department of
Stomatologic Sciences, Federal University of Goiás,
Goiânia, GO, Brazil

Júlio Almeida Silva, Federal University of Goiás

Professor of Endodontics, Department of
Stomatologic Sciences, Federal University of Goiás,
Goiânia, GO, Brazil

Daniel Almeida Decurcio, Federal University of Endodontics

Professor of Endodontics, Department of
Stomatologic Sciences, Federal University of Goiás,
Goiânia, GO, Brazil

Carlos Estrela, Federal University of Goiás

Professor of Endodontics, Department of
Stomatologic Sciences, Federal University of Goiás,
Goiânia, GO, Brazil

References

Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol. 1965;20(3):340-9. https://doi.org/10.1016/0030-4220(65)90166-0

Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85(1):86-93. https://doi.org/10.1016/S1079-2104(98)90404-8

Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99(2):231-52. https://doi.org/10.1016/j.tripleo.2004.10.005

Estrela C, Sydney GB, Figueiredo JAP, Estrela CRA. Antibacterial efficacy of intracanal medicaments on bacterial biofilm: a critical review. J Appl Oral Sci. 2009;17(1):1-7. https://doi.org/10.1590/S1678-77572009000100002

Estrela C, Holland R, Estrela CR, Alencar AH, Sousa-Neto MD, Pécora JD. Characterization of successful root canal treatment. Braz Dent J. 2014;25(1):3-11. https://doi.org/10.1590/0103-6440201302356

Sjögren U, Hägglund B, Sundqvist G, Wing K. Factors affecting the longterm results of endodontic treatment. J Endod. 1990;16:498-504. https://doi.org/10.1016/S0099-2399(07)80180-4

Sedgley C, Nagel A, Dahlén G, Reit C, Molander A. Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals. J Endod. 2006;32(3):173-77. https://doi.org/10.1016/j.joen.2005.10.037

Estrela C, Estrela CRA, Decurcio DA, Hollanda ACB, Silva JA. Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals. Int Endod J. 2007;40(2): 85 93. https://doi.org/10.1111/j.1365-2591.2006.01185.x

Estrela C, Estrela CRA, Barbin EL, Spanó JCE, Marchesan MA, Pécora JD. Mechanism of action of sodium hypochlorite. Braz Dent J. 2002; 13(2):113-7. https://doi.org/10.1590/S0103-64402002000200007

Zhu WC, Gyamfi J, Niu LN, Schoeffel GJ, Liu SY, Santarcangelo F, Khan S, Tay, K.C, Pashey DH, Tay FR. Anatomy of sodium hypochlorite a conventional irrigation dents involving facial ecchymosis – A review. J Dent. 2013;4:935-48. https://doi.org/10.1016/j.jdent.2013.08.012

Arruda AO, Souza LG, Biz MT, Ramos IFAS, Figueiredo JAP, Mazzuoo C. Análise macroscópica e MEV da superfície do canal radicular após utilização do extrato de própolis a 0,25% como irrigante. J Bras Endod. 2004;5(19):280-7.

Ramos IFAS, Biz MT, Paulino N, Scremin A, Della Bona A, Barletta FB, Figueiredo JAP. Histopathological analysis of corticosteroid antibiotic preparation and propolis paste formulation as intracanal medication after pulpectomy: an in vivo study. J Appl Oral Sci. 2012; 20(1):50-6. https://doi.org/10.1590/S1678-77572012000100010

Maia Filho EM, Maia CCR, Bastos ACSC, Novais TMG. Efeito antimicrobiano in vitro de diferentes medicações endodônticas e própolis sobre Enterococcus faecalis. Rev Gaucha Odontol. 2008;56(1):21-5.

Koo H, GOMES BPFA, Rosalen PL, Ambrosano GMB, Park YK, Cury JA. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arc Oral Biol. 2000;45:141-8. https://doi.org/10.1016/S0003-9969(99)00117-X

Castro LM, Cury AJ, Rosalyn LP. Própolis do sudeste e nordeste do brasil: influência da sazonalidade na atividade antibacteriana e composição fenólica. Quim Nova. 2007;30(7):1512-6. https://doi.org/10.1590/S0100-40422007000700003

Bankova V. Chemical diversity of propolis and the problem of standardization. J Ethnopharmacol. 2005;100:114-7. https://doi.org/10.1016/j.jep.2005.05.004

Silva BB, Rosalen PL, Cury JA, Ikegaki M, Souza VC, Esteves A, Alencar SM: Chemical com-position and botanical origin of red propolis, a new type of Brazilian propolis. Evid Based Complement Alternat Med; 2008; 5:313-6. https://doi.org/10.1093/ecam/nem059

Alencar SM, Oldoni TLC, Castro ML, Cabral ISR, Costa-Neto CM, Cury JA, Rosalen PL, Ikegaki M. Chemical composition and biological activity of a new type of Brazilian propolis: Red propolis. J Ethno-pharmacolo. 2007;10:01-06. https://doi.org/10.1016/j.jep.2007.06.005

Scazzocchio F, D’Auria FD, Alessandrini D, Pantanella F. Multifactorial aspects of antimicrobial activity of propolis. Microbiol Res. 2005;4:327-33.

Van Der Sluis LWM, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J. 2007; 40:415-26. https://doi.org/10.1111/j.1365-2591.2007.01243.x

Bhuva B, Patel S, Wilson R, Niazi S, Beighton D, Mannocci F. The effectiveness of passive ultrasonic irrigation on intraradicular Enterococcus faecalis biofilms in extracted single‑rooted human teeth. J Endod. 2010;43:241‑50. https://doi.org/10.1111/j.1365 2591.2009.01672.x

Batista LLV, Campesatto EA, Assis MLB, Barbosa APF, Girillo LAM, Dornelas CB. Comparative study of topical green and red propolis in wound repair in mice. Rev Col Bras Cirurg. 2012;39(6):515-20. https://doi.org/10.1590/S0100-69912012000600012

Duarte S, Rosalen PL, Hayacibara MF et al. The influence of a novel propolis on biofilms of Streptococcus mutans and caries development in rats. Arch Oral Biol. 2006;51:15-22. https://doi.org/10.1016/j.archoralbio.2005.06.002

Al-QathamiH, Al-Madi BDS. Comparison of sodium hypochlorite, propolis and salineas root canal irrigants: A pilot study. Saudi Dent J. 2003; 15(2):100-3.

Valera MC, Rosa JA, Maekawa LE. Action of propolis and medications against Escherichia coli and endotoxin in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110:70-4. https://doi.org/10.1016/j.tripleo.2010.01.029

Viuda-Martos M, Ruiz-Navajas Y, Fernandez-Lopez J, Pérez-Álvarez JA. Functional Properties of Honey, Propolis, and Royal Jelly. J Food Sci. 2008;73(9):117-124. https://doi.org/10.1111/j.1750-3841.2008.00966.x

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12:564-82.

Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Uskudar Guclu A, Akca E, OzkokTuylu A, Sorkun K, Tanyuksel M, Salih B. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007;13:140-5. https://doi.org/10.1016/j.anaerobe.2007.02.001

Ehsani M, Marashi MA, Zabihi E, Issazadeh M, Khafri S. A Comparison between Anti-bacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an in vitro study). Summer 2013;2(7)1-8.

Gomes RT, Teixeira KIR, Cortés ME, Santos RV. Antimicrobial activity of a propolis adhesive formulation on different oral pathogens. Braz J Oral Sci. 2007;6(22):1387-91.

Vargas AC, Loguercio AP, Witt NM, Costa MM, Silva MS, Viana LR. Atividade antimicrobiana “in vitro” de extrato alcóolico de própolis. Cien Rur. 2004;34(1):159-63. https://doi.org/10.1590/S0103-84782004000100024

Marcucci MC, Ferreres F, García-Viguera, Bankova VS, De Castro SL, Valente PHN, Dantas AP, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J Ethnopharmacol. 2001;74: 105-12. https://doi.org/10.1016/S0378-8741(00)00326-3

Só MVR, Wagner MH, Rosa RA, Telles L, Colpani F, Henz S, Magro ML. Atividade antimicrobiana in vitro de uma suspensão de própolis frente ao Enterococcus faecalis. Rev Gaúcha Odontol. 2011;16(3):277-81.

Bhardwaj A, Velmurugan N, Sumitha Ballal S. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis) in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study. Indian J Dent. 2012;24(1):35-41. https://doi.org/10.4103/0970-9290.114938

Harrison AJ, Chivatxaranukul P, Parashos P, Messer HH. The effect of ultrasonically activated irrigation on reduction of Enterococcus faecalis in experimentally infected root canals. Int Endod J. 2010,43:968-77. https://doi.org/10.1111/j.1365-2591.2010.01715.x

Downloads

Published

2017-11-23

Issue

Section

Original Article