Perfil genotípico de Enterococcus faecalis isolados de carne de frango e de infecção urinária pela técnica molecular RAPD-PCR

Authors

  • Arthur Guedes Costa Universidade Federal do Rio Grande do Sul
  • Ana Paula Guedes Frazzon Universidade Federal do Rio Grande do Sul
  • Pedro Alves D’Azevedo Universidade Federal de Ciências da Saúde de Porto Alegre
  • Jeverson Frazzon Universidade Federal do Rio Grande do Sul
  • Sueli Teresinha Van Der Sand Universidade Federal do Rio Grande do Sul

Keywords:

Enterococcus faecalis, Ferramentas moleculares, Microbiologia.

Abstract

Enterococcus faecalis are important hospital pathogens with a remarkable ability to express resistance to multiple antimicrobial agents. Their ubiquitous nature and resistance to adverse environmental conditions makes it easy to spread through the food chain. Because of this, the present study investigated the genetic variability of 38 E. faecalis strains resistant to antibiotics from chicken meat and urinary tract infection by RAPD-PCR. For this, oligonucleotide primer M13 were used to verify the existence of a genetic correlation between the isolated. The dendrogram formed from RAPD-PCR profile, generated 3 groups (A, B and C). Group A was composed of approximately 74% (28/38) clinical and food isolated with multi-resistance profile to antibiotics and with 88% of similarity. Group A was subdivided into I and II, where the sub-group I are grouped all clinical isolated and the sub-group II was composed by the majority of food isolated of E. faecalis. Group B was composed of 21% (8/38) of clinical and food isolated with 90% similarity and it was subdivided into III, IV and V. The sub-group III was formed by two strains with similar genetic profile, one from urinary tract infection (EFAE1260) resistant to erythromycin, and another from chicken meat (EFAECL52) sensitive to all antibiotics tested. In sub-group IV with 100% similarity are two samples from urinary tract infection, with profile of resistance to tetracycline, erythromycin and ciprofloxacin. Four isolated genetically identical, three from chicken meat (two sensitive to all antibiotics tested and 1 resistant to erythromycin) and 1 of urinary tract infection (multi-resistant), were grouped in the sub-group V. Group C was formed by isolated identified as Enterococcus spp. The isolated EFAEC617 and EFAECL51 joined to other isolated with 80% similarity. The results observed show that the technique of RAPD-PCR can be used for studies of genotypic species of enterococci isolated from different environments, such as food and clinical specimens. Even with the results can be seen that a route of transmission of resistant Enterococcus faecalis may be experiencing food for humans.

Author Biographies

Arthur Guedes Costa, Universidade Federal do Rio Grande do Sul

Bacharel em Farmácia pela Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.

Ana Paula Guedes Frazzon, Universidade Federal do Rio Grande do Sul

Professor do Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.

Pedro Alves D’Azevedo, Universidade Federal de Ciências da Saúde de Porto Alegre

Professor adjunto do Departamento de Microbiologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Brasil.

Jeverson Frazzon, Universidade Federal do Rio Grande do Sul

Professor do Instituto de Ciência e Tecnologia dos Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.

Sueli Teresinha Van Der Sand, Universidade Federal do Rio Grande do Sul

Professor do Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.

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Published

2010-03-03

Issue

Vol. 17 No. 1 (2009)

Section

Research Papers

License

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