Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis

Rodrigo Daniel Genske, Eduardo Alexandre Loth, Vanessa Cecatto, Rinaldo Ferreira Gandra, Cleverson Marcelo Pilatti, Maricília Silva Costa

Abstract


AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.


Keywords


Paracocidioidomycosis; Arthritis experimental; laser therapy; Receptors purinergic; Brilliant Blue G.

References


Lacaz, CS. Paracoccidioidomicose. In: Lacaz, CS, editores. Tratado de micologia médica. 9th ed. São Paulo: Sarvier; 2002.

Coutinho ZF, Silva D, Lazera M, Petri V, Oliveira RM, Sabroza PC, Wanke, B. Paracoccidioidomycosis mortality in Brazil (1980-1995). Cad Saúde Pública. 2002;18(5):1441-54.

Scheinberg MA, Cohen M, Abdalla RJ, Guidule J. Artroscopia na blastomicose articular do joelho. Rev Bras Ortop. 1994;(29):577-578.

Wanke B, Aide MA. Paracocidioidomycosis. J Bras Pneumol. 2009;35(12):1245-49.

Martinez R. Atualização no uso de agentes antifúngicos. J Bras Pneumol. 2006;32(5):449-60;

Prates RA, Silva EG, Suzuki LC, Paula CR, Ribeiro MS. Parâmetros de irradiação influenciam na inativação de leveduras tratadas com terapia fotodinâmica. Rev Bras Fis Med. 2010;4(1):53-7.

Issa MC, Manela-Azulay M. Terapia fotodinâmica: revisão da literatura e documentação iconográfica. An Bras Dermatol. 2010;85(4):501-11.

Ralevic V, Burnstock G. Receptors for purines and pyrimidines. Pharmacol Rev. 1998;50(3):413-92.

Volonté C, Apolloni S, Skaper SD, Burnstock G. P2X7 receptors: channels, pores and more. CNS Neurol Disord Drug Targets. 2012;11(6):705-21.

Loth EA, Biazin SK, Paula CR, Simão Rde C, de Franco MF, Puccia R, Gandra RF. Experimental model of arthritis induced by Paracoccidioides brasiliensis in rats. Mycopathologia. 2012;174(3):187-91.

Hansch A, Frey O, Gajda M, Susanna G, Boettcher J, Bräuer R, et al. Photodynamic treatment as a novel approach in the therapy of arthritic joints. Lasers Surg Med. 2008;40(4):265-72.

Hamblin MR. Antimicrobial photodynamic inactivation: a bright newtechnique to kill resistant microbes. Curr Opin Microbiol. 2016;33:67 73.

Ribeiro CM, Caixeta CA, Carli ML, Sperandio FF, Magalhães EM, Pereira AA, et al. Photodynamic inactivation of oral paracoccidioidomycosis affecting woman with systemic lupus erythematosus: an unusual case report. Photodiagnosis Photodyn Ther. 2017;17:160-3.

Sperandio FF, Simões A, Aranha AC, Correa L, Orsini Machado de Sousa SC. Photodynamic therapy mediated by methylene blue dye in wound healing. Photomed Laser Surg. 2010;28(5):581-7.

Peng W, Cotrina ML, Han X, Yu H, Bekar L, Blum L. Systemic administration of an antagonist of the ATP-sensitive receptor P2X7 improves recovery after spinal cord injury. Proc Natl Acad Sci U S A. 2009;106(30):12489-93.

Burnstock G, Krügel U, Abbracchio MP, Illes P. Purinergic signalling: from normal behaviour to pathological brain function. Prog Neurobiol. 2011;95(2):229-74.

Amaral EP, Ribeiro SC, Lanes VR, Almeida FM, de Andrade MR, Bomfim CC, et al. Pulmonary infection with hypervirulent mycobacteria reveals a crucial role for the P2X7 receptor in aggressive forms of tuberculosis. Plos Pathogens. 2014;10(7):e1004188.

Ratkay LG, Chowdhary RK, Neyndorff HC, Tonzetich J, Waterfield JD, Levy JG. Photodynamic therapy; a comparison with other immunomodulatory treatments of adjuvant-enhanced arthritis in MRL-lpr mice. Clin Exp Immunol. 1994;95(3):373-7.

Castano AP, Demidova TN, Hamblin NR. Mechanisms in photodynamic therapy: part one-photosensitizers, photochemistry and cellular localization. Photodiagnosis Photodyn Ther. 2004;1(4):279-93.

Cavassani KA, Tristao FS, Oliveira LL, Rocha FA, Vancim JO, Moreira AP, et al. Cell-free antigens from Paracoccidioides brasiliensis drive IL-4 producton and increase the severity of paracoccidioidomycosis. Plos One. 2011;6(6):e21423.




DOI: http://dx.doi.org/10.15448/1980-6108.2019.2.32705

This journal is a member of COPE (Committee on Publication Ethics) and follow the principles recommended by this international reference organization on integrity and ethics in scientific publication.

Licença Creative Commons
Except where otherwise noted, the material published in this journal is licensed under a Creative Commons Attribution 4.0 International licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original publication is properly cited.
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

 
 Member of OASPA

Copyright: © 2006-2019 EDIPUCRS