Cytotoxicity of current adhesive systems: in vitro testing on cell culture of L929 and balb/c 3T3 fibroblasts


  • Laiza Tatiana Poskus Universidade Federal Fluminense
  • Rosana Sampaio Meirelles Souza Lima
  • Ingrid Russoni Lima
  • José Guilherme Antunes Guimarães
  • Eduardo Moreira da Silva
  • José Mauro Granjeiro


Cytotoxicity tests, adhesives, fibroblasts, cell culture, in vitro


Purpose: The aim of this study was to evaluate the cytotoxicity of three current adhesives: Prime&Bond NT (PBNT), Single Bond (SB) and XENO III (XENO). Methods: After embedding and curing circles of filter paper with the tested adhesives, the filters were placed in contact with the solidified agar surface over L929 monolayer cells plated in 6-well cell culture plate and incubated for 24 h. The inhibition zone around the filter papers was measured in mm. MTT assay was performed using fibroblasts Balb/c 3T3 cell lines in multiwell culture plates. All assays were done in triplicate. Results: All materials were cytotoxic (Kruskal-Wallis, P < 0.05) in a similar level to latex (P > 0.05). For intra-groups analysis, SB presented the lowest cytotoxicity (P < 0.01), while there was no statistical difference between PBNT and XENO (P > 0.05). MTT assay confirmed the cytotoxicity of the tested adhesives. Conclusion: Considering the limits of this work, all adhesives tested were as cytotoxic as latex.

Author Biography

Laiza Tatiana Poskus, Universidade Federal Fluminense

Faculdade de Odontologia Departamento de Odontotécnica Disciplina de Dentística






Original Article