Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells
Keywords:Amphotericin B, cell culture, dental pulp
AbstractPurpose: The aim of the present work is to evaluate the behavior (survival and proliferation) of human third molar pulp cells cultivated in the presence or absence of amphotericin B. Methods: The cells were obtained from six impacted third molars extracted for orthodontic reasons. The pulp was minced into pieces, which were incubated at 37ºC for 60 minutes in buffer containing 0.2% collagenase. The cell suspension was divided into two tubes, centrifuged, and resuspensed in DMEM medium, supplemented with 10% fetal bovine serum, 100 units/mL penicillin, 100μg/mL streptomycin, 0.45μg/mL gentamincin, and 3.7mg/L HEPES with or without 2.5μg/mL of amphotericin B. The cells were plated in plastic culture dishes and incubated with 5% CO2/95% air at 37ºC. Results: Two out of the six tooth samples did not growth at any of the tested media. The other four samples growth in both media. The growth was more effective in the medium without amphotericin B. Conclusions: Although amphotericin B may prevent fungal contamination, it was concluded that amphotericin B may difficult the culture of human pulp cells. Key words: Amphotericin B; cell culture; dental pulp
The submission of originals to Odonto Ciência implies the transfer by the authors of the right for publication. Authors retain copyright and grant the journal right of first publication. If the authors wish to include the same data into another publication, they must cite Odonto Ciência as the site of original publication.
CREATIVE COMMONS LICENSE
As this journal is open access, the articles are allowed free use in scientific and educational applications, with citation of the source.
According to the type of Creative Commons License (CC-BY 4.0) adopted by Odonto Ciência, the user must respect the requirements below.
You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
However, only under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests Odonto Ciência endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
For more details on the Creative Commons license, please follow the link in the footer of this website.