In situ biofilm formation on enamel and glass ionomer cement in different oxygen tensions
Keywords:Biofilm, enamel, glass ionomer cement, in situ study
AbstractPurpose: To evaluate the in situ biofilm formation on enamel fragments restored with glass ionomer cement. Methods: Six volunteers wore removable intra-oral devices similar to orthodontic appliances, containing four fragments of bovine enamel restored with glass ionomer cement. A blind examiner visually analyzed the biofilm formation on enamel and glass ionomer cement specimens by using a standardized index after 24, 48, 96, and 168 hours (7 days). Results: No significant difference was detected between the biofilm formation on enamel and on glass ionomer cement. The results showed a faster biofilm development on specimens facing the palate, independently from the substrate. Conclusions: Glass ionomer cement did not affect microbial colonization. Biofilm development was higher where less oxygen was available. Key words: Biofilm; enamel; glass ionomer cement; in situ study
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