Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing

Authors

  • Mailza Costa de Almeida Federal University of Amazonas, Manaus-AM
  • Luiz Carlos de Lima Ferreira Federal University of Amazonas, Manaus-AM, Brazil
  • Gisely Naura Venâncio Federal University of Amazonas, Manaus-AM
  • Risonilce Fernandes Silva de Souza National Research Institute of Amazonas, Manaus-AM
  • Emerson Silva Lima Federal University of Amazonas, Manaus-AM
  • Nikeila Chacon de Oliveira Conde Federal University of Amazonas, Manaus-AM
  • Maria Fulgência Costa Lima Bandeira Federal University of Amazonas, Manaus-AM

DOI:

https://doi.org/10.15448/1980-6523.2017.2.26479

Keywords:

Materials testing, Propolis, Dental cavity lining, Detergents

Abstract

OBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests.
METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II.
RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II.
CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.

Author Biographies

Mailza Costa de Almeida, Federal University of Amazonas, Manaus-AM

Department of Post-Graduation, School of
Dentistry, Federal University of Amazonas, Manaus,
Brazil

Luiz Carlos de Lima Ferreira, Federal University of Amazonas, Manaus-AM, Brazil

Department of Pathology and Legal Medicine,
School of Medicine, Federal University of Amazonas,
Manaus, Brazil

Gisely Naura Venâncio, Federal University of Amazonas, Manaus-AM

Department of Post-Graduation, School of
Dentistry, Federal University of Amazonas, Manaus,
Brazil

Risonilce Fernandes Silva de Souza, National Research Institute of Amazonas, Manaus-AM

Department of Research in Health Science,
National Research Institute of Amazonas, Manaus,
Brazil

Emerson Silva Lima, Federal University of Amazonas, Manaus-AM

Department of Clinical Analysis, School of
Pharmaceutical Sciences, Federal University of
Amazonas, Manaus, Brazil

Nikeila Chacon de Oliveira Conde, Federal University of Amazonas, Manaus-AM

Department of Post-Graduation, School of
Dentistry, Federal University of Amazonas, Manaus,
Brazil

Maria Fulgência Costa Lima Bandeira, Federal University of Amazonas, Manaus-AM

Department of Post-Graduation, School of
Dentistry, Federal University of Amazonas, Manaus,
Brazil

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Published

2017-11-23

Issue

Vol. 32 No. 2 (2017)

Section

Original Article

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