Antimicrobial action of an intracanal medication trial using Aloe vera

Authors

  • Amanda Pereira Beserra UFPI
  • Débora Lima e Silva UFPI
  • Jessyca Leal Moura Fé UFPI
  • Isadora Mello Vilarinho Soares UFPI
  • Josie Haydée Lima Ferreira Paranagua UFPI
  • Carmen Milena Rodrigues Siqueira Carvalho UFPI

DOI:

https://doi.org/10.15448/1980-6523.2015.4.15717

Keywords:

Microbiology, Endodontics, Phytotherapy, Phytotherapeutic drugs, Aloe vera

Abstract

Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.

Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.

Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.

Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.

Author Biographies

Amanda Pereira Beserra, UFPI

Scientific Initiation Student, Department of Pathology and Dental Practice, Federal University of Piauí (UFPI), Teresina, PI, Brazil – 

Débora Lima e Silva, UFPI

Scientific Initiation Student, Department of Pathology and Dental Practice, Federal University of Piauí (UFPI), Teresina, PI, Brazil – 

Jessyca Leal Moura Fé, UFPI

Dentistry Master’s Student, Odontology Post-Graduate Program, Federal University of Piauí (UFPI), Teresina, PI, Brazil

Isadora Mello Vilarinho Soares, UFPI

Dentistry Master’s Student, Odontology Post-Graduate Program, Federal University of Piauí (UFPI), Teresina, PI, Brazil

Josie Haydée Lima Ferreira Paranagua, UFPI

Master's degree in Microbiology and Immunology - USP, Assistant Professor, Departament of Parasitology and Microbiology, Federal University of Piauí (UFPI), Teresina, PI, Brazil

Carmen Milena Rodrigues Siqueira Carvalho, UFPI

Doctor’s degree in Endodontics and Restorative Dentistry - UPE, Adjunct Professor, Department of Pathology and Dental Practice, Federal University of Piauí (UFPI), Teresina, PI, Brazil 

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Published

2016-01-15

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Section

Original Article