Immunoexpression of PCNA and α-SMA in gingival overgrowth tissues
DOI:
https://doi.org/10.15448/1980-6523.2015.4.15303Keywords:
Gingival overgrowth, Phenytoin, Nifedipine, Gingival fibromatosisAbstract
Purpose: This study aimed to investigate proliferating cell nuclear antigen (PCNA) and isoform-α of the smooth muscle actin (α-SMA) immunoexpression, as well as the epithelial thickness in druginduced gingival overgrowth (DIGO) and idiopathic gingival fibromatosis (IGF) patients.
Methods: Gingival samples were obtained from 11 users of nifedipine and phenytoin, 6 of patients with IGF and 4 fibrous inflammatory hyperplasia. The specimens were submitted to immunohistochemical and morphological analysis.
Results: Our results show that PCNA-epithelial positive cells were slightly more common among IGF patients, but there were not statistically significant differences among the groups (p>0.05). Comparing the vessels counts with α SMA-positive pericyte or smooth muscular cells, there was not statistical differences, although the control group presented a discrete higher count (p>0.05). The epithelial thickness analysis revealed that the DIGO group presented the higher mean, evidencing statistically significant differences in relation to the control group (p=0,002).
Conclusion: In conclusion, our results demonstrated similarities between DIGO and IGF according to PCNA and α-SMA immunoexpression, although the epithelial thickness was higher in DIGO group.
References
Shouda J, Nakamoto H, Sugahara S, Okada H, Suzuki H. Incidence of gingival hyperplasia caused by calcium antagonists in continuous ambulatory peritoneal dialysis patients. Adv Perit Dial. 1999;15: 153-5.
Bulut S, Uslu H, Ozdemir BH, Bulut OE. Analysis of proliferative activity in oral gingival epithelium in immunosuppressive medication induced gingival overgrowth. Head Face Med. 2006;2:13.
Shimizu Y, Kataoka M, Seto H, Kido J, Nagata T. Nifedipine induces gingival epithelial hyperplasia in rats through inhibition of apoptosis. J Periodontol. 2002;73:861-7.
Fu E, Nieh S, Hsiao CT, Hsieh YD, Wikesjö UM, Shen EC. Nifedipineinduced gingival overgrowth in rats: brief review and experimental study. J Periodontol. 1998;69:765-71.
Ellis JS, Seymour RA, Steele JG, Robertson P, Butler TJ, Thomason JM. Prevalence of gingival overgrowth induced by calcium channel blockers: a community-based study. J Periodontol. 1999;70:63-7.
Lucchesi JA, Cortelli SC, Rodrigues JA, Duarte PM. Severe phenytoininduced gingival enlargement associated with periodontitis. Gen Dent. 2008;56:199-203.
Kantarci A, Black SA, Xydas CE, Murawel P, Uchida Y, Yucekal-Tuncer B, et al. Epithelial and connective tissue cell CTGF/CCN2 expression in gingival fibrosis. J Pathol 2006;210:59-66.
Uzel MI, Kantarci A, Hong HH, Uygur C, Sheff MC, Firatli E, et al. Connective tissue growth factor in drug-induced gingival overgrowth. J Periodontol 2001;72:921-31.
Lobão DS, Silva LC, Soares RV, Cruz RA. Idiopathic gingival fibromatosis: a case report. Quintessence Int. 2007;38:699-704.
Akca AE, Ortakoğlu K, Pikdöken L, Deveci S. Histopathological evaluation of five unusual gingival enlargement cases. Mil Med. 2005;170:986-90.
Kantarci A, Nseir Z, Kim YS, Sume SS, Trackman PC. Loss of Basement Membrane Integrity in Human Gingival Overgrowth. J Dent Res. 2011;90:887-3.
Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some neoplasms. JPathol. 1990;162:285-94.
Kurki P, Vanderlann M, Dolbeare F, Gray J, Tan EM. Expression of proliferating cell nuclear antigen (PCNA) cyclin during the cell cycle. Exp Cell Res 1986;166:209-19.
Badid C, Mounier N, Costa AM, Desmoulière A.. Role of myofibroblasts during normal tissue repair and excessive scarring: Interest of their assessment in nephropathies. Histol Histopathol 2000;15:269-80.
Damasceno LS, Gonçalves Fda S, Costa e Silva E, Zenóbio EG, Souza PE, Horta MC. Stromal myofibroblasts in focal reactive overgrowths of the gingiva. Braz Oral Res. 2012;26:373-7.
Hinz B, Phan SH, Thannickal VJ, Galli A, Bochaton-Piallat ML, Gabbiani G. The myofibroblast: one function, multiple origins. Am J Pathol 2007;170:1807-16.
Bitu CC, Sobral LM, Kellermann MG, Martelli-Junior H, Zecchin KG, Graner E, et al. Heterogeneous presence of myofibroblasts in hereditary gingival fibromatosis. J Clin Periodontol. 2006;33:393-400.
Bonan PR, Kaminagakura E, Pires FR, Vargas PA, de Almeida OP. Histomorphometry and immunohistochemical features of Grade I (WHO) oral mucositis during radiotherapy of the head and neck cancer. Oral Dis 2007;13:170-6.
Coletta RD, Graner E. Hereditary gingival fibromatosis: A systematic review. J Periodontol 2006;77:753-64.
Bondon-Guitton E, Bagheri H, Montastruc JL. Drug-induced gingival overgrowth: a study in the French Pharmacovigilance Database. J Clin Periodontol. 2012;39:513-8.
Spolidorio LC, Spolidorio DM, Neves KA, Gonzaga HF, Almeida OP. Morphological evaluation of combined effects of cyclosporin and nifedipine on gingival overgrowth in rats. J Periodontal Res. 2002;37:192-5.
Cetinkaya BO, Acikgoz G, Aydın O, Korkmaz A, Keles GC. The Relationship between Proliferating Cell Nuclear Antigen Expression and Histomorphometrical Alterations in Cyclosporin A-Induced Gingival Overgrowth in Rats. Toxicol Pathol. 2006;34:180-6.
Castro LA, Elias LS, Oton-Leite AF, de Spíndula-Filho JV, Leles CR, Batista AC. Long-term effects of nifedipine on human gingival epithelium: a histopathological and immunohistochemical study. J Oral Sci. 2010;52:55-62.
Sobral LM, Montan PF, Martelli-Junior H, Graner E, Coletta RD. Opposite effects of TGF-β1 and IFN-γ on transdifferentiation of myofibroblast in human gingival cell cultures. J Clin Periodontol. 2007;34:397-406.
Hughes S, Gardiner T, Baxter L, Chan-Ling T. Changes in pericytes and smooth muscle cells in the kitten model of retinopathy of prematurity: implications for plus disease. Invest Ophthalmol Vis Sci. 2007;48:1368-79.
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