Microbiological evaluation of peracetic acid for disinfection of acrylic resins

  • Cheila Denise Ottonelli Stopiglia
  • Mariana Carissimi
  • Maria Lúcia Scroferneker
  • Carmen Beatriz Borges Fortes
Keywords: Peracetic acid, acrylic resins, Candida albicans


Purpose: The aim of this study was to assess the efficacy of peracetic acid (PAA) for the disinfection of dental acrylic resins experimentally contaminated with Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Fifteen materials were used for each type of resin (thermosetting, self-curing and microwave-curing). Each material was placed in a test tube containing culture medium with a suspension of each microorganism and then incubated. The materials were rinsed and transferred to other tubes containing 50 mL of water for 5 min, 0.2% peracetic acid for 5 min or glutaraldehyde for 30 min. The materials were placed in the culture agar and incubated. Microbial growth was determined by colony counting after plating. Results: Candida albicans growth was inhibited by peracetic acid and glutaraldehyde treatments. The number of colonies on resins treated with saline was greater than 105 CFU/mL. In resins infected with E. coli, S. aureus and P. aeruginosa the colony growth was not inhibited by saline and peracetic acid, but it was totally inhibited by glutaraldehyde. Conclusion: Surface disinfection using peracetic acid effectively inhibited C. albicans growth on all acrylic resins.
Original Article