Pulp tissue dissolution when the use of sodium hypochlorite and EDTA alone or associated

  • Marcus Vinicius Só UFRGS
  • Fabiana Vieira Vier-Pelisser PUCRS
  • Mirena Sonza Darcie UNICSUL
  • Daniele Geni Rockenbach Smaniotto UNICSUL
  • Francisco Montagner UFRGS
  • Milton Carlos Kuga UNESP
Keywords: Sodium hypochlorite, EDTA, pulp tissue, endodontics


Purpose: The aim of the present study was to evaluate the tissue dissolving capacity of various concentrations of sodium hypochlorite either alone or in combination with 17% EDTA. Methods: Eighty bovine pulp fragments were prepared, and their weight was determined using a precision balance. Each pulp fragment was immersed for 2 hours in a solution/mixture that was based on the following groups: G1 – saline solution; G2 – 0.5% NaOCl; G3 – 1.0% NaOCl; G4 – 2.5% NaOCl; G5 – 17% EDTA; G6 – 0.5% NaOCl + 17% EDTA; G7 – 1.0% NaOCl + 17% EDTA; and G8 – 2.5% NaOCl + 17% EDTA. The final weight was measured, and the weight loss was calculated. A statistical analysis was performed using either the Student’s t-test for paired samples or an ANOVA and Tukey tests (P < 0.05 was considered to be significant). Results: We measured a significant difference between the sample weight before and after treatment for each of the tested groups (P < 0.05). The 2.5% sodium hypochlorite solution (G4) completely dissolved the pulp tissue within the test period. NaOCl + EDTA was less effective than sodium hypochlorite alone at dissolving the pulp tissue (P < 0.05), and EDTA alone (G5) did not markedly dissolve the pulp tissue. Conclusion: Using EDTA together with NaOCl reduced the tissue dissolving properties compared with NaOCl alone, regardless of the concentration of NaOCl that was used.

Author Biographies

Marcus Vinicius Só, UFRGS
Pepartamento de Odontologia Conservadora- Área de Endodontia
Fabiana Vieira Vier-Pelisser, PUCRS
Professor Adjunto de Endodontia
Mirena Sonza Darcie, UNICSUL
Especialista em Endodontia
Daniele Geni Rockenbach Smaniotto, UNICSUL
Especialista em Endodontia
Francisco Montagner, UFRGS
Professor Adjunto I de Endodontia
Milton Carlos Kuga, UNESP
Professor Adjunto de Endodontia
Original Article