Comparison of the interface dentin-endodontic sealer using two SEM magnifications

  • Liviu Steier Warwick Medical School
  • José Antônio Poli de Figueiredo PUCRS
  • Sema Belli Selcuk University
Keywords: Sealer, dentin, SEM analysis


Purpose: The aim of this in vitro study was to compare the interface dentin-sealer of two sealers (RealSeal and AH Plus) using two magnifications under Scanning Electron Microscope (SEM). Methods: The coronal two thirds of eight extracted molars were removed and the dentin surfaces were grounded with the sequential use of #180 and #320 SIC paper under water cooling. The samples were divided into two groups of four in accordance to the sealer to be used. The dentin surfaces were treated with 17% EDTA followed by 5.25% NaOCl. Cylinders from polyethylene tubes were applied to the dentin surfaces and filled with freshly prepared sealers. Following 2h setting and storage at 37ºC at 100% humidity for a week, the teeth were cut perpendicularly to allow the visualization of the area containing the hole, thus the interface dentin-sealer. SEM analysis was done at three areas along the interface, at 150x and1000x. Results: The repeated measures ANOVA showed no significant differences between the magnifications. AH-Plus displayed significantly more gaps in the interface dentin-sealer than RealSeal (P=0.002). The increase in magnification, from 150x to 1000x, did not allow any additional identification of gaps. Conclusion: RealSeal produced less gaps than AH Plus. It could be assumed that 150x is good enough to show defects in the interface dentin-sealer.

Author Biographies

Liviu Steier, Warwick Medical School
Wepartamento de Prótese Dentária. Programa de Pós-Graduação em Odontologia. PUCRS
José Antônio Poli de Figueiredo, PUCRS
Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil
Sema Belli, Selcuk University
Department of Endodontics, Faculty of Dentistry, Selcuk University, Konya, Turkey
Original Article