Effect of two models of stress associated with ligature-induced periodontitis on hematological parameters in rats

  • Leonardo Stephan Caporossi UNIC
  • Aurélio Rosa da Silva Universidade de Cuiabá -UNIC.
  • Tereza A. Delle Vedove Semenoff UNESP - Araçatuba - SP.
  • Fábio Miranda Pedro Universidade de Cuiabá -UNIC.
  • Alvaro Henrique Borges Universidade de Cuiabá - UNIC, MT.
  • Alex Segundo Semenoff-Segundo CEOPE, UNIC e UNIVAG
Keywords: Stress, rats, periodontitis

Abstract

Purpose: To evaluate the effect of two models of chronic stress in rats and their association with induced periodontitis on hematological parameters: mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hematocrit (Ht), erythrocytes (Hm), hemoglobin (Hg) and leukocytes (Lk). Methods: Forty-eight adult Wistar rats were randomly distributed into four groups (n=12): physical stress (PSG), variable stress (VSG), ligature (LG) and control (CG) and then started the test of physical stress (restraint and exposure to cold) and variable stress (exposure to flashing light, isolation, examination of the oral cavity, congested environment, the smell of blood and noise). After 10 days of the stress test, the animals in Groups PS, VS and L were anesthetized, and a silk thread was adapted around the upper right second molar; subsequently, the stress test continued for 50 days. The animals were anesthetized and held up the incision and visualization of the posterior vena cava for blood puncture vacuum in tubes containing EDTA. Data were collected by blinded and trained examiners and were statistically analyzed by means of ANOVA and Bonferroni’s test at the significance level of 0.05. Results: The two models of stress changed all of the hematological parameters tested, with the exception of VCM. Conclusion: The stress associated with periodontitis is able to modify blood parameters in rats.

Author Biographies

Leonardo Stephan Caporossi, UNIC
Graduando em Odontologia pela Universidade de Cuiabá -MT.
Aurélio Rosa da Silva, Universidade de Cuiabá -UNIC.
Graduando em Odontologia pela Universidade de Cuiabá -MT.
Tereza A. Delle Vedove Semenoff, UNESP - Araçatuba - SP.
Doutora em Estomatologia pela UNESP - Araçatuba,SP.
Fábio Miranda Pedro, Universidade de Cuiabá -UNIC.
Diretor da Faculdade de Odontologia de Cuiabá - UNIC, MT.
Alvaro Henrique Borges, Universidade de Cuiabá - UNIC, MT.
Professor de Endodontia e Odontologia em âmbito Hospitalar da Universidade de Cuiabá - UNIC,MT.
Alex Segundo Semenoff-Segundo, CEOPE, UNIC e UNIVAG
Centro Estadual de Odontologia para pacientes Especiais - CEOPE, Universidade de Cuiabá -UNIC- e Centro universitário de Várzea Grande - UNIVAG.
Published
2010-09-25
Section
Original Article