Cytotoxicity of carbamide peroxide bleaching gel on L929 cells

  • Rogério Laceda Santos UFRJ
  • Matheus Melo Pithon UFRJ
  • Fernanda Otaviano Martins UFRJ
  • Maria Teresa Villela Romanos UFRJ
Keywords: Toxicity, hydrogen peroxide, dental bleaching

Abstract

Purpose: To test the hypothesis that the higher the concentration of carbamide peroxide, the greater is its cytotoxicity to fibroblast cells. Methods: Three concentrations of carbamide peroxide (10%, 16%, and 22%) used in home bleaching techniques were evaluated regarding their cytotoxic effect on gingival tissues. The materials were divided into three groups as follows: Group C10 (White Gold Home 10%, Dentsply), Group C16 (White Gold Home 16%, Dentsply, and Group C22 (Nite White 22%, ACP Discus Dental). The cytotoxicity essay was carried out using cell cultures (mouse fibroblast L929 cell line) in which the viable cells were determined by means of the dye-uptake method performed at 2, 4, and 8 hours. Data were analyzed by analysis of variance (ANOVA) with multiple comparisons and Tukey’s test (P < 0.05). Results: The results showed statistically significant differences between Groups C10, C16, C22, and the cell control at 2, 4, and 8 hours (P < 0.05). The amount of cell lysis increased proportionally to the exposure time to the materials studied. Conclusion: The 22% carbamide peroxide group was more toxic than the other two groups (16% and 10% concentration) regardless of the exposure time.

Author Biographies

Rogério Laceda Santos, UFRJ
Especialista em Ortodontia pela Universidade Federal de Alfenas – UNIFAL/MG, Minas Gerais, Brasil Mestre e Doutorando em Ortodontia pela Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, Brasil
Matheus Melo Pithon, UFRJ
Especialista em Ortodontia pela Universidade Federal de Alfenas – UNIFAL/MG, Minas Gerais, Brasil Mestre e Doutorando em Ortodontia pela Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, Brasil
Fernanda Otaviano Martins, UFRJ
Graduada em Ciências Biológicas: Microbiologia e Imunologia pela Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, Brasil
Maria Teresa Villela Romanos, UFRJ
Professora Assistente de Microbiologia da Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, Brasil
Published
2010-06-23
Section
Original Article