Effect of saliva contamination on the bond strength of an etch-and-rinse adhesive system to dentin

  • Luciana Neves Guerriero Universidade de São Paulo
  • Samuel Nilo Vieira Universidade de São Paulo
  • Taís Scaramucci Universidade de São Paulo
  • Fernando Aparecido Kawaguchi Universidade de São Paulo
  • Maria Angela Pita Sobral Universidade de São Paulo
  • Adriana Bona Matos Universidade de São Paulo
Keywords: Bond strength, adhesion, saliva contamination

Abstract

Purpose: The aim of this study was to investigate the effect of saliva contamination on bond strength of an etch-and-rinse system to dentin. Methods: Fifty bovine incisors were embedded in acrylic resin and divided into 5 groups: G1 (control) – application of the adhesive system (Adper Single Bond 2 – 3M-ESPE); G2 – saliva contamination after acid etching of dentin, rinsing and drying; G3 – saliva contamination after acid etching of dentin and drying; G4 – saliva contamination after adhesive application, rinsing and drying; G5 – saliva contamination after adhesive application and drying. Contamination was performed by using 4 μL of simulated human saliva for 20 s. The adhesive system was applied according to the manufacturer’s instructions; a composite resin was built as an inverted cone and was tested after 24 h at a cross-head speed of 0.5 mm/min. Results: When saliva contamination occurred after the adhesive photo-polymerization, bond strength was significantly reduced. The adhesive strength (MPa) mean values were: G1 = 18.1(±4.7) a; G2 = 20.5(±5.7) a; G3 = 17.3(±3.4) a; G4 = 12.6(±4.0) b; G5 = 9.8(±2.1) b (means followed by distinct letters are statistically different, P < 0.05). Conclusion: Saliva contamination negatively influenced bond strength of an etch-and-rinse adhesive, especially after the final polymerization of the adhesive system; in this condition, treatments were not efficient to recover adhesion.

Author Biographies

Luciana Neves Guerriero, Universidade de São Paulo
Cirurgiã-dentista. Formada pela Faculdade de Odontologia da Universidade de São Paulo
Samuel Nilo Vieira, Universidade de São Paulo
Estudante de graduação da Faculdade de Odontologia da Universidade de São Paulo.
Taís Scaramucci, Universidade de São Paulo
Doutoranda do Departamento de Dentística da Faculdade de Odontologia da Universidade de São Paulo.
Fernando Aparecido Kawaguchi, Universidade de São Paulo
Doutorando do Departamento de Dentística da Faculdade de Odontologia da Universidade de São Paulo.
Maria Angela Pita Sobral, Universidade de São Paulo
Professora Assistente do Departamento de Dentística da Faculdade de Odontologia da Universidade de São Paulo.
Adriana Bona Matos, Universidade de São Paulo
Professora Associada do Departamento de Dentística da Faculdade de Odontologia da Universidade de São Paulo.
Published
2009-08-15
Section
Original Article