Cytotoxicity of dental alginates

  • Matheus Melo Pithon Universidade Federal do Rio de Janeiro-UFRJ
  • Rogério Lacerda dos Santos Universidade Federal do Rio de Janeiro-UFRJ
  • Fernanda Otaviano Martins Universidade Federal do Rio de Janeiro-UFRJ
  • Maria Teresa Villela Romanos Universidade Federal do Rio de Janeiro-UFRJ
Keywords: Citotoxicidade, Materiais para Moldagem Odontológica, Técnicas de Cultura de Células.


Purpose: To evaluate the cytotoxicity of dental alginate (irreversible hydrocolloid), which is widely used as an impression material in Dentistry. Methods: Four dental products were assessed: J (Jeltrate Traditional), ALG (Alga Gel), PG (Printer Gel), and AVG (Ava Gel). Three control groups were used: positive (C+) cell detergent Tween 80, negative (C–) PBS, and control of cells (CC – no exposure of cells to any substance). Disk-shaped specimens were immersed in Eagle minimum essential. The supernatants were collected after 24, 48, 72, and 168 hours (7 days) for analysis of the toxicity to L929 fibroblast cells after 24-h incubation. Viable cells stained with 0.01% neutral red dye were counted using a spectrophotometer. Data were analyzed by ANOVA and Tukey’s test (α=0.05). Results: Significant differences in number of viable cells were found between the alginate groups and C– or CC (P < 0.05). The group J showed the highest cytotoxicity level followed by PG, ALG, and AVG. Conclusion: All dental alginates tested showed some cytotoxic response from fibroblasts.
Original Article