In vitro evaluation of the cytotoxicity of palatal expanders
AbstractObjective: To evaluate the cytotoxicity of two palatal expanders made of stainless steel or composite. Methods: Six expanders were divided into two experimental groups: metallic expander and composite expander. Three control groups also were assessed: positive control (amalgam), negative control (glass stick), and control cell (cells not exposed to any material). The sterilized expanders were immersed into Eagle’s minimum essential medium for 24 h, and the contact assay was performed using L929 fibroblasts. The cells were incubated for 24 h, and 100 μL of 0.01% neutral-red staining solution were added followed by incubation for 3 h and cell fixation. Cytotoxicity was evaluated at four different periods of time: 24, 48, 72, and 168 h. Counting of viable cells was performed by using a spectrophotometer at a wavelength of 492 nm, and data were analyzed by ANOVA. Results: The positive control groups (amalgam) were statistically different from the other groups. No difference of cytotoxicity was found among the groups and periods of time. Conclusions: The tested metallic and composite expanders showed no significant cytotoxicity within this experimental design.
The submission of originals to Odonto Ciência implies the transfer by the authors of the right for publication. Authors retain copyright and grant the journal right of first publication. If the authors wish to include the same data into another publication, they must cite Odonto Ciência as the site of original publication.
CREATIVE COMMONS LICENSE
As this journal is open access, the articles are allowed free use in scientific and educational applications, with citation of the source.
According to the type of Creative Commons License (CC-BY 4.0) adopted by Odonto Ciência, the user must respect the requirements below.
You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
However, only under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests Odonto Ciência endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
For more details on the Creative Commons license, please follow the link in the footer of this website.