Effect of chronic stress on the repair of cutaneous wounds in rats


  • Alex Semenoff Segundo UNIC
  • Tereza A. Delle Vedove Semenoff UNESP
  • Eder Ricardo Biasoli
  • Samyra Lopes Buzelle UFMT
  • Suellen Lara Guirra Rosa UFMT


stress, wound healing, rats


Purpose: The aim of this study was to determine the effect of different types of chronic stress on the contraction of cutaneous wounds on the back of rats. Methods: Nineteen rats were randomly divided into two groups: chronic stress - GCS (n=9) and control – GC (n=10). The GCS were subjected to testing of chronic stress (exposure to light flashing, isolation, crowded environment, noise and smell of blood) throughout the experiment for a period of 10 days. The animals were anesthetized, and a surgical wound with area of 1 cm2 was made on the back of each animal, preserving the muscle tissue. The measurements of the wounds were made by a blinded examiner at 3, 7 and 12 days after surgery, using a digital caliper. The contraction of the wound was measured using the following formula: (initial area – area on the day of measurement) / initial area × 100 = percentage of wound contraction. Data were analyzed by Student t test for independent samples. Results: The results showed a lower rate of wound contraction in stressed animals at 7 and 12 days after surgery in comparison with the control group. Conclusion: Based on the methods and the animal model used, it was possible to conclude that rats subjected to chronic stress have delayed wound contraction during repair.

Author Biographies

Alex Semenoff Segundo, UNIC

Professor Doutor da Universidade de Cuiabá – Odontologia em âmbito hospitalar e Periodontista do Centro de Especialidades Odontológicas para Pacientes Especiais – CEOPE/MT

Tereza A. Delle Vedove Semenoff, UNESP

Doutoranda do Programa de Pós-Graduação em Odontologia Área de Concentração Estomatologia da UNESP - Campus Araçatuba.

Samyra Lopes Buzelle, UFMT

Mestranda em Biociências da Universidade Federal do Estado de Mato Grosso

Suellen Lara Guirra Rosa, UFMT

Mestranda em Biociências da Universidade Federal do Estado de Mato Grosso






Original Article