Sex estimation study in skulls from Brazil’s southeastern population using Physical Anthropology and DNA
AbstractOBJECTIVE: This study aimed to determine the sex in human craniums using methodologies of Physical Anthropology, quantitative (Forensic Data Anthropology Bank, FDB, 1986) and one qualitative (Walker, 2008) and genetic analysis by amelogenin.
METHODS: The sample was composed of 66 skulls (34 males and 32 females) from the Center for Study and Research in Forensic Science, Guarulhos, SP. The methodologies were applied by two researchers who were unaware of the cranium’s sexes. For the statistical analysis, there were performed descriptive analysis, average, standard deviation, linear discriminant analysis and logistic regression.
RESULTS: The qualitative methodology presented an accuracy of 89.52%. For the DNA, it was possible to determine the sex in 86.15% of the sample. Analyzing the results for each skull in three different methodologies, we reached 100% correct.
CONCLUSION: As a result of this study, it is recommended that physical anthropology be the chosen method if it presents good accuracy when applied to different populations or if it is validated for the analyzed population. Otherwise, genetic analysis should be used for the determination of the sex.
Alvesalo L. Human sex chromosomes in oral and craniofacial growth, Archives of Oral Biology 2009;54(1):18-24. https://doi.org/10.1016/j.archoralbio.2008.06.004
Buikstra JE, Ubelaker DH. Standards for data collection from human skeletal remains. Proceedings of a seminar at the Field Museum of Natural History, organized by Jonathan Haas. Fayetteville, AK: Arkansas Archeological Survey; 1994. p. 206.
Chang YM, Perumal R, Keat PY, Yong RY, Kuehn DL, Burgoyne L. A distinct Y-STR haplotype for Amelogenin negative males characterized by a large Yp11.2 (DYS458-MSY1-AMEL-Y) deletion. Forensic Sci Int 2007;166:115-20. https://doi.org/10.1016/j.forsciint.2006.04.013
Dayal MR, Spocter MA, Bidmos MA. An assessment of sex using the skull of black South Africans by discriminant function analysis. HOMO 2008; 59:209-21. https://doi.org/10.1016/j.jchb.2007.01.001
Franklin D, Freedman L, Milne N. Sexual dimorphism and discriminant function sexing in indigenous South African crania. HOMO 2005;55:213-28. https://doi.org/10.1016/j.jchb.2004.08.001
Ganswindt M, Ehrlich E, Klostermann P, Troike WG, Schneider V. Bone finds: a challenge to forensic science. Legal Medicine 2003;5:382-85. https://doi.org/10.1016/S1344-6223(02)00137-2
Giles E, Elliot O. Sex determination by discriminant function analysis of crania. Am J Phys Anthrop 1963;21:53-68. https://doi.org/10.1002/ajpa.1330210108
H ill CR, Butler JM, Vallone PM. A 26plex autosomal STR assay to aid human identity testing. J Forensic Sci 2009; 54 (5): 1008-15. https://doi.org/10.1111/j.1556-4029.2009.01110.x
Moore-Jansesn et al. FDB - Forensic Anthropology Data Bank. Tennesse: University of Tennessee; 1986. Departament of Anthropology. [Acesso em Aug. 10. 2010]. Disponível: http://fac.utk.edu/databank.html
Konigsberg LW, Hens SM. Use of ordinal categorical variables in skeletal assessment of sex from the cranium. Am J of Physical Anthrop 1998;107: 97-112. https://doi.org/10.1002/(SICI)1096-8644(199809)107:1<97::AIDAJPA8> 3.0.CO;2-A
Rogers T. Determining the sex of human remains through cranial morphology. J Forensic Sci. 2005;50(3):1-8. https://doi.org/10.1520/JFS2003385
Saini V, Srivastava R, Rai RK, Shamal SN, Singh TB, Tripathi SK. An Osteometric Study of Northern Indian Populations for Sexual Dimorphism in Craniofacial Region. J Forensic Sc In ternational 2011;56(3):700-5. https://doi.org/10.1111/j.1556-4029.2011.01707.x
Williams BA, Rogers TL. Evaluating the accuracy and precision of cranial morphological traits for sex determination. J Sci Forense 2006;51:729-35. https://doi.org/10.1111/j.1556-4029.2006.00177.x
Walker PL. Sexing skulls using dicriminant function analysis of visually assessed traits. Am J Phys Anthropol 2008;136:39-50. https://doi.org/10.1002/ajpa.20776
Sweet D, Hildebrand D. Recovery of DNA from human teeth by cryogenic grinding. J Forensic Sci 1998; 43(6): 1199-202. https://doi.org/10.1520/JFS14385J
Kemp BM, Smith DG. Use of bleach to eliminate contaminating DNA form the surface of bones and teeth. Forensic Sci Int 2005;154:53-61. https://doi.org/10.1016/j.forsciint.2004.11.017
Johnson DR, O’Hihhins P, Moore WJ, McAndrew TJ. Determination of race and sex of the human skull by dicriminant function analysis of linear and angular dimensions. Forensic Sci Int. 1989;41:41-53. https://doi.org/10.1016/0379-0738(89)90234-X
Uytterschaut HT. Sexual dimorphism in human skulls. A comparison of sexual dimorphism in different populations. Human Evolution 1986;1(3):243-50. https://doi.org/10.1007/BF02436582
Oliveira RN, Daruge E, Daruge JR, Galvão LCC. Determinação do sexo através de mensurações mandibulares. Revista da Associação Bras. de Odontol. 1995;3:241-44.
Spradley MK, Jantz RL. Sex Estimation in Forensic Anthropology: Skull Versus Postcranial Elements. J Forensic Sci 2011;56(2):289-96. https://doi.org/10.1111/j.1556-4029.2010.01635.x
Santos JFM. Análise da amelogenina pulpar para determinação do gênero biológico [tese]. Piracicaba (SP): Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba; 2012.
V elarde-Félix JS, Molina-Benítez CE, Solórzano-Rosales SR, Cázarez- Salazar SG, Rendón-Aguilar H, Murillo-Llanes J, Ríos-Tostado JJ. Identificación del sexo mediante análisis molecular. Rev Mex Patol Clin 2008;55(1):17-20.
Tschentscher F, Frey UH, Bajanowski T. Amelogenin sex determination by pyrosequencing of short PCR products. Int J Legal Med 2008;122:333-35. https://doi.org/10.1007/s00414-008-0228-4
Ricaut FX, Tarcqui CK, Crubézy E, Ludes B. STR-genotyping form human medieval tooth and bone samples. Forensic Sci Int 2005;151:31-5. https://doi.org/10.1016/j.forsciint.2004.07.001
Kashyap VK, Sahoo S, Sitalaximi T, Trivedi R. Deletions in the Y-derived amelogenin gene fragment in the Indian population, BMC Med Genet 2006;7:37. https://doi.org/10.1186/1471-2350-7-37
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