Ex vivo analysis of marginal apical sealing ability of a mta Fillapex®

  • Nilce Mendes Faria Pinto Pontifícia Universidade Católica de Minas
  • Maria Eugênia Alvarez-Leite Pontifícia Universidade Católica de Minas
  • Eduardo Nunes Pontifícia Universidade Católica de Minas
  • Martinho Campolina Rebello Horta Pontifícia Universidade Católica de Minas
  • Héctor Michel Rodrigues Pontifícia Universidade Católica de Minas
  • Frank Ferreira Silveira Pontifícia Universidade Católica de Minas
Keywords: Dental cements, Root canal obturation, Root canal therapy


Objective: The present ex vivo study aimed to assess the apical sealing ability of the endodontic sealer MTA FILLAPEX® compared to Sealapex, Pulp Canal Sealer, and AH26.
Methods: The ability to prevent leakage of a culture of Enterococcus faecalis through the root canal obturation was assessed. Forty-eight single-rooted teeth that had been extracted from humans were used. Following instrumentation and obturation using the warm gutta-percha vertical condensation technique, the teeth were allocated into one of four experimental groups (n=10), a positive control group (n=4), or a negative control group (n=4). The microbial inoculation was performed every three days over 60 days. The data were tabulated and subjected to statistical survival analysis, whereby the performance of the four investigated sealers was compared using the log-rank test.
Results: The results revealed that all of the samples in the group in which MTA FILLAPEX® was used exhibited leakage. Sealapex and Pulp Canal Sealer exhibited leakage in 90 and 80% of the samples, respectively. The sealer AH26 was significantly superior in delaying leakage, although 30% of the samples exhibited leakage.
Conclusion: MTA FILLAPEX® permitted the most Enterococcus faecalis leakage compared to the other investigated sealers.


Saunders W, Saunders E. Coronal leakage as a cause of failure in rootcanal therapy: a review. Endod Dent Traumatol. 1994; 10:105-8.

Schilder H. Cleaning and shapping the root canal. Dent Clin North Am. 1974; 18:269-96.

Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod. 1999; 26:197-205.

Camilleri, J. Hydration mechanisms of mineral trioxide aggregate. Int Endod J. 2007; 40:462-70.

Camilleri, J. Characterization of hydration products of mineral trioxide aggregate. Int Endod J. 2008; 41:408-17.

Valadares MAA, Soares JA, Nogueira C C, Cortes MIS, Leite MEA, Nunes E. et al. The efficacy of a cervical barrier in preventing microleakage of Enterococcus faecalis in endodontically teeth. Gen Dent. 2011; 59:e32-7.

Kokkas AB, Boutsioukis ACH,Vassiliadis LP, Stavrianos CK. The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: an in vitro study. J Endod. 2004; 30:100-2.

Dultra F, Barroso JM, Carrasco LD, Capelli A, Guerisoli DMZ, Pecora JD. Evaluation of apical microleakage of teeth sealed with four differents root canal sealers. J Appl Oral Sci. 2006;14:341-5.

Camps J, Pashley D. Reability of the dye penetration studies. J Endod. 2003; 29:592-4.

Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990; 16:566-9 .

Chailertvanitkul P, Saunders WP, Mackenzie D. Coronal leakage of obturated root canals after long-term storage using a polymicrobial marker. J Endod. 1997; 23: 610-3.

Pisano DM, Difiori PM, Mcclanahan SB, Lautenschlager EP, Ducan JL. Intraorifice sealing of gutta-percha obturated root Canals to prevent coronal microleakage. J Endod. 1998; 24: 659-62.

Wolcott JF, Hicks ML, Himel VT. Evaluation of pigmented intraorifice barrires in endodontically treated teeth. J Endod. 1999; 25: 589-92.

Nup C, Boylan R, Ippolito G, Ahn SH, Erakin C, Rosenberg PA. An evaluation of resin-ionomers to prevent coronal microleakage in endodontically treated teeth. The J Clin Dent. 2000; 11: 16-9.

Siqueira Júnior JF, Roças IN, Favieri A, Abadi EC, Castro AJR, Gayva SM. Bacterial leakage in coronal unsealed root canals obturated with 3 different techniques. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000;90:647-50.

Wolanek GA, Loushine RJ, Weller RN, Kimbrough WF, Volkmann KR. In vitro bacterial penetration of endodoncally treated teeth coronally sealed with a dentin bonding agent. J Endod. 2001;27:354-7.

Tselnik M, Baumgartner JC, Marshall JG. Bacterial leakeage with mineral trioxide aggregate or a resin-modified glass ionomer used as a coronal

barrier. J Endod. 2004;30:782-4.

Chailertvanitkul P, Saunders WP, Mackenzie D, Weetman DA. An in vitro

study of the coronal leakage of two root canal sealers using an obligate

anaerobe microbial marker. Int Endod J. 1996;29:249-55.

Sunde PT, Olsen I, Debelian GJ, Tronstad L. Microbiota of Periapical Lesions Refractory to Endodontic Therapy. J Endod. 2002;28:304-10.

Abid V, Spratt D, Gulabivala K, Y.-L Ng. Cultivable microbial flora associated with persistent periapical disease and coronal leakage after root canal treatment: a preliminary study. Int Endod J. 2004;37:542-51.

Saleh IM, Ruyter IE, Haapasalo M, Orstavik D. Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers in vitro. Int Endod J. 2004;37:193-8.

Grecca FS, Rosa AR, Gomes, MS, Parolo, CF, Bemfica JR, Frasca LC. et al. Effect of timing and method of post space preparation on sealing ability of remaining root filling material: in vitro microbiological study. J Can Dent Assoc. 2009;5:583.

Almeida J, Gomes BP, Ferraz CC, Souza-Filho FJ, Zaia AA. Filling of artificial lateral canals and microleakage and flow of five endodontic sealers. Int Endod J. 2007;40:692-9.

Bodrumlu E, Tunga U. The apical sealing ability of a new root canal filling material. Am J Dent. 2007;20:295-8.

De Moor RJ, De Bruyne MA. The long-term sealing ability of AH 26 and AH plus used with three gutta-percha obturation techniques. Quintessence Int. 2004;35:326-31.

Original Article