Surface roughness influences Candida albicans biofilm formation on denture materials

  • Wander José da Silva Piracicaba Dental School, State University of Campinas
  • Letícia Machado Gonçalves Piracicaba Dental School, State University of Campinas
  • Flávia Carvalho Viu Piracicaba Dental School, State University of Campinas
  • Cristiane Maria Brasil Leal Piracicaba Dental School, State University of Campinas
  • Célia Marisa Rizatti Barbosa Piracicaba Dental School, State University of Campinas
  • Altair Antoninha Del Bel Cury Piracicaba Dental School, State University of Campinas
Keywords: Acrylic resins, Biofilms, Candida albicans


Objective: This study aimed to evaluate the influences of surface roughness (SR) of denture base and liner materials on Candida albicans biofilm formation.
Methods: Discs were fabricated using poly (methyl methacrylate) acrylic resin and poly(ethyl methacrylate) denture liner, according to manufacturers’ instructions. Both acrylic resin and relined discs were finished or finished + polished and the SR was measured. Discs were saliva-coated, 
and C. albicans biofilms were developed on such surfaces for 48 hours. Biofilms were evaluated for cell counts, metabolic activity, and structural characteristics. Data were analyzed by one-way ANOVA and Tukey tests using a significance level of 5%.
Results: Finished acrylic resin and denture liner discs had higher SR compared to finished + polished discs (P<0.001). Finished + polished acrylic resin discs contained fewer cells compared to only finished discs (P<0.001). No metabolic activity differences were seen between biofilms formed on both groups (P>0.05). Bulky, thick and less rough biofilms were formed on only finished denture materials (P<0.05). Confocal images reveal increased presence of black spaces for biofilms developed on finished + polished discs. Conclusion: Reduced SR resulted in decreased C. albicans biofilm accumulation on both denture materials.


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Original Article