Potentiation of the action of calcium hydroxide on Enterococcus faecalis by proton pump inhibitor omeprazole

  • Deborah Meirelles Cogo Pontifical Catholic University of Rio Grande do Sul
  • Sílvia Dias de Oliveira Federal University of Rio Grande do Sul
  • Fernanda Camargo Antunes Federal University of Rio Grande do Sul
  • Patrícia Maria Poli Kopper Pontifical Catholic University of Rio Grande do Sul
  • Jéssica Stephanie Rodrigues Nasário Federal University of Rio Grande do Sul
  • Fabiana Vieira Vier-Pelisser Pontifical Catholic University of Rio Grande do Sul
Keywords: In vitro, Bacteria, Enterococcus faecalis, Calcium hydroxide, Omeprazole.

Abstract

Purpose: Calcium hydroxide is not fully effective against Enterococcus faecalis (E. faecalis), a facultative anaerobic bacterium proven to be resistant to most conventional disinfection processes. The aim of this in vitro study was to evaluate the effect of calcium hydroxide, omeprazole and the association of these substances against Enterococcus faecalis, as well as to evaluate if the acidcatalysation of the omeprazole had any influence in the results.
Methods: The Minimum Inhibitory Concentration (MIC) of these drugs against E. faecalis (ATCC 29212) was determined using macrodilution test adapted from the CLSI (Clinical Laboratory and Standards Institute). Solutions with different concentrations of calcium hydroxide, associated or not to omeprazole, were tested. Data were statistically analyzed using ANOVA test with Tukey post-hoc, with a level of significance of 5%.
Results: The MIC to calcium hydroxide was 32 mg mL-1 and, when associated with omeprazole, this was reduced reduced to 16 mg mL-1. The omeprazole and acidified omeprazole had similar activity. 
Conclusions: Omeprazole potentiated the effect of calcium hydroxide, since the association of these drugs reduced the MIC for E. faecalis. The acidification of omeprazole, when associated with calcium hydroxide in different concentrations, did not influence its effect.

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Published
2015-08-18
Section
Original Article