Comparison of immunoexpression of VEGF, TGF-β and MMP-9 in ameloblastoma and adenomatoid odontogenic tumor

  • Stefânia Jeronimo Ferreira Federal University of Rio Grande do Norte
  • Emeline das Neves de Araújo Lima Federal University of Rio Grande do Norte
  • Conceição Aparecida D. Monteiro Maia Federal University of Rio Grande do Norte
  • Fábio Henrique de Sá Leitão Pinheiro Potiguar University
  • Roseana de Almeida Freitas Federal University of Rio Grande do Norte
  • Hébel Cavalcanti Galvão Federal University of Rio Grande do Norte
Keywords: Odontogenic tumors, Immunohistochemistry, Vascular endothelial growth factor (VEGF), Matrix metalloproteinase 9 (MMP-9), Transforming growth factor β (TGF-β).

Abstract

Purpose: Studies on odontogenic tumors have identified various molecular dysfunctions involved on their development, and some mechanisms such as angiogenesis and matrix modulation, are useful means of investigating the differences in biologic behavior of these tumors. Some important markers to identify tumor aggressiveness by immunohistochemistry are VEGF, TGF-β and MMP-9 proteins. This study aimed to compare the immunohistochemical expression of VEGF, TGF-β, and MMP-9 in ameloblastoma and adenomatoid odontogenic tumor (AOT).
Methods: Immunoexpression of VEGF, TGF-β, and MMP-9 was studied in 15 solid ameloblastomas, and 15 AOTs. A semi-quantitative analysis of the immunostained cells was performed, and the statistical analysis was made using the Mann-Whitney nonparametric and Spearman correlation test, with significance level at .05 (P<.05).
Results: A higher epithelial immunoexpression of VEGF and TGF-β was observed in ameloblastoma and AOT, respectively, and the stromal reactivity to VEGF was statistically higher in ameloblastoma (P<.05). No statistical difference was observed for MMP-9 (P>.05).
Conclusion: The results suggest the involvement of angiogenesis in tumor progression of ameloblastomas, and the inductive effect of stromal cells in AOT, hence justifying its lower growth potential.

Author Biographies

Stefânia Jeronimo Ferreira, Federal University of Rio Grande do Norte
PhD Student, Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil
Emeline das Neves de Araújo Lima, Federal University of Rio Grande do Norte
PhD Student, Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil
Conceição Aparecida D. Monteiro Maia, Federal University of Rio Grande do Norte
PhD Student, Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil
Fábio Henrique de Sá Leitão Pinheiro, Potiguar University
PhD, Professor, Potiguar University, Natal, Brazil
Roseana de Almeida Freitas, Federal University of Rio Grande do Norte
PhD, Professor, Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil
Hébel Cavalcanti Galvão, Federal University of Rio Grande do Norte
PhD, Professor, Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil

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Published
2015-09-01
Section
Original Article