Influence of location and storage time in cohesive strength of dentin

  • Stéfani Becker Rodrigues UFRGS
  • Vicente Castelo Branco Leitune UFRGS
  • Susana Maria Werner Samuel UFRGS
  • Fabrício Mezzomo Collares UFRGS
Keywords: Dentin, tensile strength, collagen, dentin-bonding agents,


Objective: The purpose of this study was to evaluate the influence of the time of storage in water at the microtensile cohesive strength of occlusal and cervical dentin. Methods: Thirty sound human molars were used. The occlusal surface was removed under water irrigation. Two slices of dentin (occlusal and cervical) of 1.0 mm of thickness were obtained. Each slice was sectioned in the mesio distal direction to obtain 5 sticks. The sticks were stored in distilled water at 37°C for 24 hours or 6 months according to the groups (occlusal and cervical). The ultimate tensile strength test was performed at a crosshead speed of 1 mm/min. Cohesive strengths were calculated in MPa. The values of cohesive strength were analyzed by two-way ANOVA and Student-Newman-Keuls, at the 5% of level of significance. Results: No significant interactions among location of dentin, P=0.59. Statistical significant difference was observed between cohesive strength of 24 hours to 6 months of storage (P=0.018). Conclusion: Influence of the time of storage in water on the microtensile cohesive strength was observed. The storage of dentin in distilled water for 6 months reduces the values of cohesive strength. However no influence of dentin location was found.

Author Biographies

Stéfani Becker Rodrigues, UFRGS
Vicente Castelo Branco Leitune, UFRGS
Susana Maria Werner Samuel, UFRGS
Fabrício Mezzomo Collares, UFRGS


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Original Article