Use of cone beam tomography to evaluate intracanal medications in a rat model of apical periodontitis

  • Larissa Magnus Klassmann Doutorado Odontologia PUCRS
  • Vinicius Dutra Radiology Service, Mãe de Deus Hospital
  • Lisiane Bernardi Postgraduate Program in Dentistry,UFRGS
  • Maria Martha Campos
Keywords: Apical periodontitis, intracanal medication, cone beam, rats,


Objective: To describe a methodology for evaluating the efficacy of intracanal medications in rats, by using cone beam computed tomography (CBCT) to follow-up periapical lesions. Methods: Six male Wistar rats were used, and periapical lesions were induced in 12 upper molars. The endodontic treatment was performed in all teeth and calcium hydroxide paste was applied in the right molars (treated group, n=6), whereas no medication was used in left molars (control group, n=6). CBCT was performed 21 days after endodontic treatment, and the lesion area was determined in mm2. Unpaired Student t test was used to verify the differences between groups. Results: The lesion area was 9.38±0.68 mm2 in the control group and 7.08±0.44 mm2 in the treated group (P<0.05), according to CBTC evaluation. These data were confirmed by histological evaluation of maxillas, indicating the applicability of CBTC in the rat model of apical periodontitis. Conclusion: The rat model and CBCT was found to be a useful tool to study in vivo the effects of intracanal medications and their influence in periapical lesions healing.

Author Biography

Larissa Magnus Klassmann, Doutorado Odontologia PUCRS
Area Endodontia


Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol. 1965;20:340-9.

Moller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89:475-84.

Gondim JO, Avaca-Crusca JS, Valentini SR, Zanelli CF, Spolidorio DM,

Giro EM. Effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against Porphyromonas gingivalis and Enterococcus faecalis. Int J Paediatr Dent. 2012;22:116-24.

Panzarini SR, Trevisan CL, Brandini DA, Poi WR, Sonoda CK, Luvizuto ER, et al. Intracanal dressing and root canal filling materials in tooth replantation: a literature review. Dent Traumatol. 2012;28:42-8.

Taneja S, Kumari M. Use of triple antibiotic paste in the treatment of large periradicular lesions. J Investig Clin Dent. 2012;3:72-6.

Riccitiello F, Stabile P, Amato M, Rengo S, D’Ambrosio C. The treatment of the large periradicular endodontic injury. Minerva Stomatol. 2011;60: 417-26.

Sathorn C, Parashos P, Messer H. Antibacterial efficacy of calcium

hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J. 2007;40:2-10.

Basrani B, Tjaderhane L, Santos JM, Pascon E, Grad H, Lawrence

HP, et al. Efficacy of chlorhexidine- and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:618-24.

Cotton TP, Geisler TM, Holden DT, Schwartz SA, Schindler WG.

Endodontic applications of cone-beam volumetric tomography. J Endod. 2007;33:1121-32.

Kim TS, Caruso JM, Christensen H, Torabinejad M. A comparison of conebeam computed tomography and direct measurement in the examination of the mandibular canal and adjacent structures. J Endod. 2010;36:1191-4.

Michetti J, Maret D, Mallet JP, Diemer F. Validation of cone beam

computed tomography as a tool to explore root canal anatomy. J Endod. 2010;36:1187-90.

Patel S, Dawood A, Ford TP, Whaites E. The potential applications of cone beam computed tomography in the management of endodontic problems. Int Endod J. 2007;40:818-30.

Lee YL, Hong CY, Kok SH, Hou KL, Lin YT, Chen MH, et al. An extract of green tea, epigallocatechin-3-gallate, reduces periapical lesions by inhibiting cysteine-rich 61 expression in osteoblasts. J Endod. 2009;35:206-11.

Sobrinho AP, Barros MH, Nicoli JR, Carvalho MA, Farias LM, Bambirra EA, et al. Experimental root canal infections in conventional and germ-free mice. J Endod. 1998;24:405-8.

Metzger Z, Klein H, Klein A, Tagger M. Periapical lesion development in rats inhibited by dexamethasone. J Endod. 2002;28:643-5.

Alshwaimi E, Purcell P, Kawai T, Sasaki H, Oukka M, Campos-Neto A, et al. Regulatory T cells in mouse periapical lesions. J Endod. 2009;35:1229-33.

Fouad A, Barry J, Russo J, Radolf J, Zhu Q. Periapical lesion progression with controlled microbial inoculation in a type I diabetic mouse model. J Endod. 2002;28:8-16.

Garlet TP, Fukada SY, Saconato IF, Avila-Campos MJ, da Silva TA, Garlet GP, et al. CCR2 deficiency results in increased osteolysis in experimental periapical lesions in mice. J Endod. 2010;36:244-50.

Wagner C, Barth VC, Jr., de Oliveira SD, Campos MM. Effectiveness of the proton pump inhibitor omeprazole associated with calcium hydroxide as intracanal medication: an in vivo study. J Endod. 2011;37:1253-7.

Burgener B, Ford AR, Situ H, Fayad MI, Hao JJ, Wenckus CS, et al.

Biologic markers for odontogenic periradicular periodontitis. J Endod. 2010;36:1307-10.

Holland R, Mazuqueli L, de Souza V, Murata SS, Dezan Junior E, Suzuki P. Influence of the type of vehicle and limit of obturation on apical and periapical tissue response in dogs’ teeth after root canal filling with mineral trioxide aggregate. J Endod. 2007;33:693-7.

Penesis VA, Fitzgerald PI, Fayad MI, Wenckus CS, BeGole EA, Johnson BR. Outcome of one-visit and two-visit endodontic treatment of necrotic teeth with apical periodontitis: a randomized controlled trial with one-year evaluation. J Endod. 2008;34:251-7.

Trope M, Delano EO, Orstavik D. Endodontic treatment of teeth with apical periodontitis: single vs. multivisit treatment. J Endod. 1999;25:345-50.

Waltimo T, Trope M, Haapasalo M, Orstavik D. Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing. J Endod. 2005;31:863-6.

Peters LB, Wesselink PR. Periapical healing of endodontically treated teeth in one and two visits obturated in the presence or absence of detectable microorganisms. Int Endod J. 2002;35:660-7.

Original Article