In vitro study of dose-response relationship of fluoride with dental enamel

  • Rodrigo Alex Arthur
  • Magda Giancristófaro
  • Cínthia Pereira Machado Tabchoury
  • Jaime Aparecido Cury


In vitro models for evaluation of fluoride (F) reactivity should present dose-response effect. Thus, the aim of this study was to evaluate the fluoride dose-response present in aqueous solution with bovine dental enamel. A hundred and twenty bovine enamel slabs (5 × 5 × 2 mm), 60 sound and 60 with caries-like lesions, were subjected during 10 min to distilled and deionized water (negative control) and aqueous solutions containing 50, 100, 200 or 400 μg F/mL. Each experimental group received 12 sound and 12 carious slabs. Two consecutive layers of dental enamel were removed from all slabs, by acid etching, and fluoride extracted was determined with specific electrode. The results of fluoride incorporated were expressed in μg per g of removed enamel, considering the total amount of the two layers. Fluoride uptake by sound enamel showed a linear dose-response (p = 0.0001), while that by carious slabs showed a polynomial one (p < 0.0001). The results suggest that the in vitro model of reactivity used in the present study is appropriate to evaluate dose-response between fluoride present in aqueous solution and that incorporated by either sound or carious bovine dental enamel.
UNITERMS: fluoride; dental enamel; reactivity; dose-response; in vitro study.
Original Article